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https://hdl.handle.net/2440/102144
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Type: | Journal article |
Title: | A primer extension denaturing high-performance liquid chromatography method for the identification of three ABCC2 genetic polymorphisms |
Author: | Westley, I. Coller, J. Ward, M. Evans, A. Morris, R. Sallustio, B. |
Citation: | Journal of Liquid Chromatography and Related Technologies, 2014; 37(9):1249-1256 |
Publisher: | Taylor and Francis |
Issue Date: | 2014 |
ISSN: | 1082-6076 1520-572X |
Statement of Responsibility: | Ian S. Westley, Janet K. Coller, Michael B. Ward, Allan M. Evans, Raymond G. Morris, and Benedetta C. Sallustio |
Abstract: | Background: A number of single nucleotide polymorphisms have been described in the ABCC2 gene that alters drug disposition. The aim of the study was to develop a primer extension denaturing high-performance liquid chromatography (PE-dHPLC) assay to determine three common variants of the ABCC2 gene in the promoter region (−24C>T), exon 10 (1249G>A), and exon 28 (3972C>T). Methods: Polymerase chain reactions (PCRs) were used to isolate the area of interest in the ABCC2 gene. A comparison of the PCR product was performed between sequencing and dHPLC. Results: A 100% identity match was achieved between groups and allowed for a quick and accurate method to determine three single nucleotide polymorphisms in a single extension reaction. This assay is the first of its type to determine three ABCC2 variants by dHPLC |
Keywords: | ABCC2; genotype; polymerase chain reaction; polymorphisms; PE-dHPLC; single nudeotide polymorphism |
Rights: | Copyright © Taylor & Francis Group, LLC |
DOI: | 10.1080/10826076.2013.789796 |
Published version: | http://dx.doi.org/10.1080/10826076.2013.789796 |
Appears in Collections: | Aurora harvest 7 Medical Sciences publications |
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