Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/104681
Citations
Scopus Web of Science® Altmetric
?
?
Type: Theses
Title: Molecular phylogeography and climate change biology of the invasive green marine macroalgae Caulerpa taxifolia and Caulerpa cylindracea in Australia
Author: Grant, William Maxwell
Issue Date: 2015
School/Discipline: School of Biological Sciences
Abstract: Populations of the green marine macroalgae Caulerpa taxifolia and Caulerpa cylindracea have invaded and spread throughout Mediterranean after they were introduced from Australia. In Australia, these tropical to subtropical species have established invasive populations in New South Wales, South Australia, and most recently, for C. cylindracea, in Victoria. Significant efforts have been made to elucidate the invasion history and geographic source locations of Mediterranean populations. The same effort has been lacking in Australia. Both species have provided challenges for molecular ecologists because of their predominantly clonal reproduction and low genetic variation within invasive populations in the case of C. taxifolia, and very high intra-individual genetic variation in C. cylindracea. In chapter 2, I present a detailed review of the literature about the molecular ecology of C. taxifolia and C. cylindracea. I outline the phylogenetic and phylogeographic research on both of these species. The methodology and analysis of each study is critiqued. From this analysis I identify the knowledge gaps about invasive Caulerpa spp., and how to approach further research given advances in technology and knowledge of the organisms. In chapter 3, I present a modified Ion Torrent next generation sequencing protocol that was used to identify novel single nucleotide polymorphisms (SNPs) in C. taxifolia. This approach resulted in the successful identification of a suite of SNPs. Primer development and SNP validation was performed for the Sequenom MassArray, and 184 specimens from 10 Australian populations from Queensland, New South Wales, South Australia, and Western Australia, were genotyped. The data build on existing phylogeographic data for C. taxifolia in Australia, and support a model of anthropogenic distribution rather than natural dispersal. Anthropogenically mediated primary introductions may have been caused by aquarium releases, while secondary spread has been facilitated by heavy boat traffic in affected areas. Finally I discuss the evidence of the so called “invasive strain” of C. taxifolia, and conclude that there is no such thing, and that the assumption that an “invasive strain” exists has undermined the scientific objectivity of 30 years of study of invasive Caulerpa species. Chapter 4 represents the first phylogeographic study of invasive and native C. cylindracea populations in Australia. In this chapter I used variation in the rpl16-rps3 region of the chloroplast to test hypotheses about the origins and dispersal mechanisms responsible for the establishment of invasive populations of C. cylindracea in South Australia. Molecular data does not support natural dispersal of C. cylindracea, a finding that is supported by a lack of species records of C. cylindracea for 2600 km between southern Western Australia and the South Australia. In chapter 5, I determined if RNA:DNA, Protein:DNA, and Protein:RNA ratios can be used to quantify the effect of ocean warming and acidification on C. taxifolia and C. trifaria. Protein profiles were also examined for effects of ocean warming and acidification. While no significant effects were observed on the ratios, concentrations of DNA were positively affected by temperature, while negative impacts on protein were associated with decreasing pH. The protein profiles also allowed me to determine if the invasive tropical and subtropical C. taxifolia is likely to be impacted differently by ocean warming and acidification than the native temperate C. trifaria. Protein profile data revealed that C. trifaria specimens experience greater levels of metabolic stress than C. taxifolia at lower pH, and that the invasive C. taxifolia will continue to thrive in warmer and more acidic ocean conditions while the native temperate species will be impacted negatively, possibly resulting in localized extinctions. In chapter 6, I discuss how new techniques and their application aid in understanding the phylogeogaphy of invasive Caulerpa spp. in Australia, paving the way for continued phylogeographic analyses of these problematic species. I have also shown that the response of native and invasive Caulerpa spp. to climate change scenarios indicates that invasive populations of Caulerpa may become more abundant and continue to expand their invasive range in the future. I also identify the limitations of this body of work and the issues encountered in the program of research, and discuss future research possibilities for invasive Caulerpa species.
Advisor: Gurgel, C. Frederico D.
Deveney, Martin Robert
Tanner, Jason
Dissertation Note: Thesis (Ph.D.) (Research by Publication) -- University of Adelaide, School of Biological Sciences, 2015.
Keywords: Caulerpa taxifolia
Caulerpa cylindracae
invasive
phylogeography
population genetics
climate change
molecular ecology
comparative proteomics
Research by Publication
Provenance: This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legals
DOI: 10.4225/55/59004be012d84
Appears in Collections:Research Theses

Files in This Item:
File Description SizeFormat 
01front.pdf59.83 kBAdobe PDFView/Open
02whole.pdf5.11 MBAdobe PDFView/Open
PermissionsLibrary staff access only180.86 kBAdobe PDFView/Open
RestrictedLibrary staff access only5.16 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.