Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/106115
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Type: | Journal article |
Title: | Efficient chromosomal-scale DNA looping in Escherichia coli using multiple DNA-looping elements |
Author: | Hao, N. Sneppen, K. Shearwin, K. Dodd, I. |
Citation: | Nucleic Acids Research, 2017; 45(9):5074-5085 |
Publisher: | Oxford University Press |
Issue Date: | 2017 |
ISSN: | 0305-1048 1362-4962 |
Statement of Responsibility: | Nan Hao, Kim Sneppen, Keith E. Shearwin, Ian B. Dodd |
Abstract: | Genes are frequently regulated by interactions between proteins that bind to the DNA near the gene and proteins that bind to DNA sites located far away, with the intervening DNA looped out. But it is not understood how efficient looping can occur when the sites are very far apart. We develop a simple theoretical framework that relates looping efficiency to the energetic cost and benefit of looping, allowing prediction of the efficiency of single or multiple nested loops at different distances. Measurements of absolute loop efficiencies for Lac repressor and λ CI using gene expression reporters in Escherichia coli cells show that, as predicted by the model, long-range DNA looping between a pair of sites can be strongly enhanced by the use of nested DNA loops or by the use of additional protein-binding sequences. A combination of these approaches was able to generate efficient DNA looping at a 200 kb distance. |
Keywords: | Infarction; lacunar; DNA; escherichia coli; tissue factor pathway inhibitor |
Rights: | The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
DOI: | 10.1093/nar/gkx069 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1100651 http://purl.org/au-research/grants/arc/DE150100091 |
Published version: | http://dx.doi.org/10.1093/nar/gkx069 |
Appears in Collections: | Aurora harvest 3 Genetics publications |
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hdl_106115.pdf | Published version | 547.81 kB | Adobe PDF | View/Open |
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