Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/111669
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Type: | Journal article |
Title: | Structural variations in wheat HKT1;5 underpin differences in Na+ transport capacity |
Author: | Xu, B. Waters, S. Byrt, C. Plett, D. Tyerman, S. Tester, M. Munns, R. Hrmova, M. Gilliham, M. |
Citation: | Cellular and Molecular Life Sciences, 2018; 75(6):1133-1144 |
Publisher: | Springer |
Issue Date: | 2018 |
ISSN: | 1420-682X 1420-9071 |
Statement of Responsibility: | Bo Xu, Shane Waters, Caitlin S. Byrt, Darren Plett, Stephen D. Tyerman, Mark Tester, Rana Munns, Maria Hrmova, Matthew Gilliham |
Abstract: | An important trait associated with the salt tolerance of wheat is the exclusion of sodium ions ( Na⁺) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na⁺- exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (Km) for the Na⁺ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D⁴⁷¹/a gap and D⁴⁷⁴/ G⁴⁷³ that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na⁺ exclusion in wheat that leads to an improved salinity tolerance in the field. |
Keywords: | Gatekeeper cells; salt exclusion; ion transport; structure-function; einkorn; bread; salt tolerance; Xenopus; mutagenesis; yeast; high-affinity K⁺ transporter |
Rights: | © Springer International Publishing AG, part of Springer Nature 2017 |
DOI: | 10.1007/s00018-017-2716-5 |
Grant ID: | http://purl.org/au-research/grants/arc/DP120100900 http://purl.org/au-research/grants/arc/CE140100008 http://purl.org/au-research/grants/arc/FT130100709 http://purl.org/au-research/grants/arc/DE150100837 |
Published version: | http://dx.doi.org/10.1007/s00018-017-2716-5 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 8 |
Files in This Item:
File | Description | Size | Format | |
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hdl_111669.pdf | Submitted Version | 1.81 MB | Adobe PDF | View/Open |
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