Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/11335
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dc.contributor.author | Shooter, G. | - |
dc.contributor.author | Magee, B. | - |
dc.contributor.author | Soos, M. | - |
dc.contributor.author | Francis, G. | - |
dc.contributor.author | Siddle, K. | - |
dc.contributor.author | Wallace, J. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Journal of Molecular Endocrinology, 1996; 17(3):237-246 | - |
dc.identifier.issn | 0952-5041 | - |
dc.identifier.issn | 1479-6813 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11335 | - |
dc.description.abstract | Insulin-like growth factor-I (IGF-I) analogues were produced with the aim of identifying IGF-I residues that contribute to the specificity of binding to the type 1 IGF receptor as opposed to the insulin receptor. Receptor binding properties of a series of A- and B-domain analogues were compared using rat L6 myoblasts, soluble human IGF type 1 receptors and soluble human insulin receptor isoforms HIR-A (−Ex11) and HIR-B (+Ex11). IGF-I analogues, [Leu8] IGF-I and [Phe59] IGF-I, were shown to exhibit respectively, a 28- and 17-fold decrease in affinity for the HIR-A with only a 6- and 5-fold decrease in affinity for the human IGF type 1 receptor. In contrast, the analogue [His4] IGF-I was equipotent to IGF-I in binding to the soluble type 1 IGF receptor while showing 7-fold and 4-fold increases in HIR-A and HIR-B binding respectively. Furthermore, [Leu62] IGF-I was 8-fold less potent than IGF-I in soluble IGF type 1 receptor binding but only showed a 2-fold decrease in HIR-A and HIR-B binding. Our study supports the conclusion that the co-evolution of the IGF-I and insulin receptor/ligand systems has resulted in subtle structural differences in the A- and B-regions of each ligand important for defining receptor binding specificity. | - |
dc.description.statementofresponsibility | Shooter, G K ; Magee, B ; Soos, M A ; Francis, G L ; Siddle, K ; Wallace, J C | - |
dc.language.iso | en | - |
dc.publisher | Bioscientifica | - |
dc.source.uri | http://dx.doi.org/10.1677/jme.0.0170237 | - |
dc.title | Insulin-like growth factor (IGF)- 1 A- and B-domain analogues with altered type 1 IGF and insulin receptor binding specificities | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1677/jme.0.0170237 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 2 Biochemistry publications |
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