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https://hdl.handle.net/2440/11408
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Type: | Journal article |
Title: | The molecular organisation of a B chromosome tandem repeat sequence from Brachycome dichromosomatica |
Author: | Franks, T. Houben, A. Leach, C. Timmis, J. |
Citation: | Chromosoma: biology of the nucleus, 1996; 105(4):223-230 |
Publisher: | SPRINGER VERLAG |
Issue Date: | 1996 |
ISSN: | 0009-5915 1432-0886 |
Statement of Responsibility: | Franks, T. ; Houben, A. ; Leach, C. ; Timmis, J. |
Abstract: | A high copy, tandemly repeated, sequence (Bd49) specific to the B chromosome and located near the centromere in Brachycome dichromosomatica was used to identify lambda genomic clones from DNA of a 3B plant. Only one clone of those analysed was composed entirely of a tandem array of the B-specific repeat unit. In other clones, the Bd49 repeats were linked to, or interspersed with, sequences that are repetitious and distributed elsewhere on the A and B chromosomes. One such repetitious flanking sequence has similarity to retrotransposon sequences and a second is similar to chloroplast DNA sequences. Of the four separate junctions analysed of Bd49-like sequence with flanking sequence, three were associated with the same A/T-rich region in Bd49 and the fourth was close to a 25 bp imperfect dyadic sequence. No novel B-specific sequences were detected within the genomic clones. |
Keywords: | Chromosomes Plants Deoxyribonucleases, Type II Site-Specific DNA, Plant Blotting, Southern Cloning, Molecular Polymerase Chain Reaction Sequence Analysis, DNA Base Sequence Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Molecular Sequence Data |
DOI: | 10.1007/BF02528770 |
Published version: | http://dx.doi.org/10.1007/bf02528770 |
Appears in Collections: | Aurora harvest 7 Genetics publications |
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