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https://hdl.handle.net/2440/11457
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Type: | Journal article |
Title: | Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica |
Author: | Donald, T. Leach, C. Clough, A. Timmis, J. |
Citation: | Heredity, 1995; 74(5):556-561 |
Publisher: | Oliver & Boyd |
Issue Date: | 1995 |
ISSN: | 0018-067X 1365-2540 |
Statement of Responsibility: | Tamzin M Donald, Carolyn R Leach, Angela Clough and Jeremy N Timmis |
Abstract: | Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status. |
Keywords: | Chromosomes Plants RNA, Plant RNA, Ribosomal Blotting, Southern In Situ Hybridization, Fluorescence Methylation |
DOI: | 10.1038/hdy.1995.77 |
Published version: | http://dx.doi.org/10.1038/hdy.1995.77 |
Appears in Collections: | Aurora harvest 7 Genetics publications |
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