Adelaide Research & Scholarship
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https://hdl.handle.net/2440/11535
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| Type: | Journal article |
| Title: | Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press |
| Author: | Jorgensen, L. O'Neill, B. Thomas, C. Morona, R. Middelberg, A. |
| Citation: | Biotechnology Letters, 1995; 9(7):477-480 |
| Publisher: | SPRINGER |
| Issue Date: | 1995 |
| ISSN: | 0951-208X 1573-6784 |
| Organisation: | Cooperative Research Centre for Tissue Growth and Repair |
| Statement of Responsibility: | Lene Jorgensen, Brian K. O'Neill, Connor J. Thomas, Renato Morona and Anton P. J. Middelberg |
| Abstract: | The release of chloramphenicol acetyl transferase (CAT) from a recombinant Escherichia coli strain by ultrasonication and the French press was compared. French pressing disrupted all cells in suspension whereas only a fraction of the cells was disrupted following sonication. The level of CAT released was highest when cells were totally disrupted. Additional treatment with the detergent Triton X-100 was necessary to maximize CAT recovery, presumably due to association of CAT with cellular debris. |
| DOI: | 10.1007/BF00159561 |
| Published version: | http://dx.doi.org/10.1007/bf00159561 |
| Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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