Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/11539
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Smith, W. | - |
dc.contributor.author | Guida, L. | - |
dc.contributor.author | Qiuy, S. | - |
dc.contributor.author | Korpelainen, E. | - |
dc.contributor.author | van den Hueven, C. | - |
dc.contributor.author | Gillis, D. | - |
dc.contributor.author | Hawrylowicz, C. | - |
dc.contributor.author | Vadas, M. | - |
dc.contributor.author | Lopez, A. | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Blood, 1995; 86(10):3938-3944 | - |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.issn | 1528-0020 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11539 | - |
dc.description.abstract | Freshly isolated peripheral blood neutrophils, unlike monocytes and eosinophils, do not bind interleukin-3 (IL-3) or respond to IL-3). We show that neutrophils cultured for 24 hours in granulocyte-macrophage colony-stimulating factor (GM-CSF) express mRNA for the IL-3 receptor (R) alpha subunit, as shown by RNase protection assays, and IL-3R alpha chain protein, as shown by cytometric analysis using two different specific monoclonal antibodies. This effect was selective for GM-CSF, because granulocyte colony-stimulating factor, tumor necrosis factor- alpha, interferon-gamma, and IL-1 failed to induce the IL-3 receptor. Saturation binding curves with 125I-IL-3 and Scatchard transformation showed the presence of about 100 high-affinity and 4,000 low-affinity receptors. Because neutrophils have been shown to express human leukocyte antigen (HLA)-DR in response to GM-CSF, we examined the possibility that IL-3 could augment HLA-DR expression on GM-CSF-treated cells. We found that neutrophils incubated with 30 ng/mL IL-3 as well as 0.1 ng/mL GM-CSF expressed a mean of 2.1-fold higher levels of HLA- DR than with GM-CSF alone (P < .005), confirming the signaling competence of the newly expressed IL-3R. This increase was seen even at maximal concentrations of GM-CSF and IL-3 can have an additive effect on mature human cells. The augmentation of HLA-DR by IL-3 was specific because it could be inhibited by a blocking anti-IL-3R antibody. Expression of class II molecules by neutrophils under these conditions may have significance for antigen presentation. These results provide further evidence for the role of GM-CSF as an amplification factor in inflammation by inducing neutrophil responsiveness to IL-3 produced by T cells or mast cells. | - |
dc.description.statementofresponsibility | WB Smith, L Guida, Q Sun, EI Korpelainen, C van den Heuvel, D Gillis, CM Hawrylowicz, MA Vadas, and AF Lopez | - |
dc.language.iso | en | - |
dc.publisher | American Society of Hematology | - |
dc.rights | © 1995 by The American Society of Hematology | - |
dc.source.uri | http://www.bloodjournal.org/content/86/10/3938 | - |
dc.subject | Neutrophils | - |
dc.subject | Cells, Cultured | - |
dc.subject | Humans | - |
dc.subject | Granulocyte-Macrophage Colony-Stimulating Factor | - |
dc.subject | Interleukin-3 | - |
dc.subject | Receptors, Granulocyte-Macrophage Colony-Stimulating Factor | - |
dc.subject | Receptors, Interleukin-3 | - |
dc.subject | Recombinant Proteins | - |
dc.subject | RNA, Messenger | - |
dc.subject | HLA-D Antigens | - |
dc.subject | Flow Cytometry | - |
dc.subject | Signal Transduction | - |
dc.subject | Neutrophil Activation | - |
dc.subject | Gene Expression Regulation | - |
dc.title | Neutrophils activated by granulocyte-macrophage colony-stimulating factor express receptors for interleukin-3 which mediate class II expression | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1182/blood.v86.10.3938.bloodjournal86103938 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Smith, W. [0000-0001-9640-1172] [0000-0002-4610-998X] | - |
dc.identifier.orcid | Lopez, A. [0000-0001-7430-0135] | - |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.