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https://hdl.handle.net/2440/115589
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Type: | Journal article |
Title: | In vitro characterization and identification of potential substrates of a low molecular weight protein tyrosine phosphatase in Streptococcus pneumoniae |
Author: | Ahmad, Z. Morona, R. Standish, A. |
Citation: | Microbiology, 2018; 164(4):697-703 |
Publisher: | Microbiology Society |
Issue Date: | 2018 |
ISSN: | 1350-0872 1465-2080 |
Statement of Responsibility: | Zuleeza Ahmad, Renato Morona and Alistair J. Standish |
Abstract: | Streptococcus pneumoniae is a major human pathogen responsible for significant mortality and morbidity worldwide. Within the annotated genome of the pneumococcus lies a previously uncharacterized protein tyrosine phosphatase which shows homology to low molecular weight protein tyrosine phosphatases (LMWPTPs). LMWPTPs modulate many processes critical for the pathogenicity of a number of bacteria including capsular polysaccharide biosynthesis, stress response and persistence in host macrophages. Here, we demonstrate that Spd1837 is indeed a LMWPTP, by purifying the protein, and characterizing its phosphatase activity. Spd1837 showed specific tyrosine phosphatase activity, and it did not form higher order oligomers in contrast to many other LMWPTPs. Substrate-trapping assays using the wild-type and the phosphatase-deficient Spd1837 identified potential substrates/interacting proteins including major metabolic enzymes such as ATP-dependent-6-phosphofructokinase and Hpr kinase/phosphorylase. Given the tight association between the bacterial basic physiology and virulence, this study hopes to prompt further investigation of how the pneumococcus controls its metabolic flux via the LMWPTP Spd1837. |
Keywords: | Tyrosine phosphorylation; low molecular weight phosphatase; pneumococcus; Streptococcus pneumoniae; phosphatase substrates identification |
Rights: | © 2018 The Authors |
DOI: | 10.1099/mic.0.000631 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1048749 |
Published version: | http://dx.doi.org/10.1099/mic.0.000631 |
Appears in Collections: | Aurora harvest 8 Molecular and Biomedical Science publications |
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