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https://hdl.handle.net/2440/11610
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dc.contributor.author | Morona, J. | - |
dc.contributor.author | Morona, R. | - |
dc.contributor.author | Paton, J. | - |
dc.date.issued | 1997 | - |
dc.identifier.citation | Journal of Bacteriology, 1997; 179(15):4953-4958 | - |
dc.identifier.issn | 0021-9193 | - |
dc.identifier.issn | 1098-5530 | - |
dc.identifier.uri | http://hdl.handle.net/2440/11610 | - |
dc.description.abstract | We have previously reported the nucleotide sequence of the Streptococcus pneumoniae type 19F capsular polysaccharide synthesis locus (cps19f), which consists of 15 open reading frames (ORFs) designated cps19fA to -O. Hybridization analysis indicated that close homologs for cps19fA to -H and cps19fK to -O were found in type 19B, but there were no homologs for cps19fI and -J. In this study we used long-range PCR to amplify and clone a 10.5-kb section of the S. pneumoniae type 19B capsule locus (cps19b) between cps19bH and cps19bK. This region of the cps19b locus is 4 kb larger than that in the cps19f locus and replaces cps19fI and cps19fJ with five new ORFs, designated cps19bP, -I, -Q, -R, and -J. We have proposed functions for four of the protein products, including functional homologs of Cps19fI and Cps19fJ. Transformation of a S. pneumoniae mutant containing an interrupted type 19F capsule locus with the 10.5-kb cps19b PCR product converted the recipient strain to type 19B. Southern hybridization analysis indicated that cps19bP, -I, -Q, -R, and -J are unique to type 19B and the closely related type 19C. | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.source.uri | http://dx.doi.org/10.1128/jb.179.15.4953-4958.1997 | - |
dc.subject | Streptococcus pneumoniae | - |
dc.subject | Bacterial Capsules | - |
dc.subject | Chromosome Mapping | - |
dc.subject | Transformation, Bacterial | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Base Sequence | - |
dc.subject | Sequence Homology, Amino Acid | - |
dc.subject | Genes, Bacterial | - |
dc.subject | Molecular Sequence Data | - |
dc.title | Molecular and Genetic Characterization of the Capsule Biosynthesis Locus Of Streptococcus pneumoniae Type 19B | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/jb.179.15.4953-4958.1997 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Morona, J. [0000-0002-4891-4122] | - |
dc.identifier.orcid | Morona, R. [0000-0001-7009-7440] | - |
dc.identifier.orcid | Paton, J. [0000-0001-9807-5278] | - |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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