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Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/11616
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dc.contributor.authorBeardsley, A.-
dc.contributor.authorGowans, E.-
dc.contributor.authorBurrell, C.-
dc.contributor.authorMarmion, B.-
dc.date.issued1996-
dc.identifier.citationJournal of Clinical Microbiology, 1996; 34(6):1581-1582-
dc.identifier.issn0095-1137-
dc.identifier.issn1098-660X-
dc.identifier.urihttp://hdl.handle.net/2440/11616-
dc.description.abstractA reverse transcription-PCR assay which successfully amplified hepatitis C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragment of the 5' untranslated region of the viral genome.-
dc.description.statementofresponsibilityA M Beardsley, E J Gowans, C J Burrell and B P Marmion-
dc.language.isoen-
dc.publisherAMER SOC MICROBIOLOGY-
dc.source.urihttp://dx.doi.org/10.1128/jcm.34.6.1581-1582.1996-
dc.subjectHumans-
dc.subjectHepacivirus-
dc.subjectMoloney murine leukemia virus-
dc.subjectViremia-
dc.subjectHepatitis C-
dc.subjectRNA-Directed DNA Polymerase-
dc.subjectDNA, Complementary-
dc.subjectRNA, Viral-
dc.subjectSensitivity and Specificity-
dc.subjectPolymerase Chain Reaction-
dc.subjectVirology-
dc.subjectBase Sequence-
dc.subjectEvaluation Studies as Topic-
dc.titleEnhanced amplification of hepatitis C virus (HCV) cDNA by PCR: Detection of HCV RNA in archival sera-
dc.typeJournal article-
dc.identifier.doi10.1128/jcm.34.6.1581-1582.1996-
pubs.publication-statusPublished-
dc.identifier.orcidGowans, E. [0000-0002-4274-8311]-
dc.identifier.orcidBurrell, C. [0000-0002-4020-349X]-
Appears in Collections:Aurora harvest 7
Microbiology and Immunology publications

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