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https://hdl.handle.net/2440/11621
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Type: | Journal article |
Title: | CD34+ cells and their derivatives contain mRNA for CD4 and human immunodeficiency virus (HIV) co-receptors and are susceptible to infection with M- and T-tropic HIV. |
Author: | Carr, J. Ramshaw, H. Li, P. Burrell, C. |
Citation: | Journal of General Virology, 1998; 79(1):71-75 |
Publisher: | SOC GENERAL MICROBIOLOGY |
Issue Date: | 1998 |
ISSN: | 0022-1317 1465-2099 |
Abstract: | Highly purified (>98%) CD34+ cells directly after isolation (D0) or 2 weeks in culture (D14) were CD4+ and contained mRNA for the T-tropic HIV co-receptor, CXCR-4, and minor co-receptor, CCR-2B. D14 but not D0 cells were RT-PCR positive for mRNA for the major M-tropic human immunodeficiency virus (HIV) co-receptor, CCR-5, and potential co-receptor, CCR-1. D14 and D0 cells were susceptible to T- (HXB2) and M-tropic HIV (Bal), showing greater virus production with Bal than HXB2, and with higher virus production levels in D14 compared to D0 cells. Seven days post-infection of D0 cells Bal DNA was present in CD14bright and CD14- fractions, suggesting D0 infection of diverse progenitor types. HXB2 DNA was detected in CD14bright cells alone indicating D0 infection of monocyte progenitors only. It is concluded that CD34+ cells and cultured derivatives are susceptible to M- and T-tropic HIV and this correlates in part with co-receptor expression at the mRNA level. |
Keywords: | Hematopoietic Stem Cells Humans HIV Receptors, Chemokine Receptors, CCR5 Receptors, CXCR4 Receptors, Cytokine Receptors, HIV Gene Products, gag HIV Core Protein p24 DNA, Viral RNA, Messenger Antigens, CD Antigens, CD34 Receptors, CCR1 Receptors, CCR2 CD4 Antigens |
DOI: | 10.1099/0022-1317-79-1-71 |
Published version: | http://dx.doi.org/10.1099/0022-1317-79-1-71 |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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