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https://hdl.handle.net/2440/117578
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Type: | Journal article |
Title: | Specific growth conditions induce a Streptococcus pneumoniae non-mucoidal, small colony variant and determine the outcome of its co-culture with Haemophilus influenzae |
Author: | Tikhomirova, A. Trappetti, C. Standish, A. Zhou, Y. Breen, J. Pederson, S. Zilm, P. Paton, J. Kidd, S. |
Citation: | Pathogens and Disease, 2018; 76(7) |
Publisher: | Oxford University Press |
Issue Date: | 2018 |
ISSN: | 2049-632X 2049-632X |
Statement of Responsibility: | Alexandra Tikhomirova, Claudia Trappetti, Alistair J Standish, Yiwen Zhou James Breen, Stephen Pederson, Peter S Zilm, James C Paton, Stephen P Kidd |
Abstract: | Haemophilus influenzae and Streptococcus pneumoniae are known aetiologic agents of chronic otitis media, frequently as a multispecies infection. In this study, we show that the outcome of H. influenzae/S. pneumoniae interactions is dependent on the nutrient source. In continuous culture containing chemically defined media with lactose, S. pneumoniae was non-viable in mono-culture, and in co-culture remained non-viable until 288 h. With glucose, S. pneumoniae became non-viable in mono-culture, but uniquely existed in 3 distinct states in co-culture: parental cells (until 24 h), a dormant state until 336 h and its re-emergence as a non-mucoidal, small colony variant (SCV). The S. pneumoniae SCV was stable and whole genome sequencing showed three major single nucleotide polymorphisms in the SCV cells-cap3A (capsule biosynthesis pathway), fpg (DNA glycosylase of the DNA repair mechanism) and glutamate-5-kinase. Previously, fpg mutants have shown increased mutator rates, permitting bacterial survival against host-generated stresses. Transcriptomics showed these SCV cells up-regulated sugar transporters and toxin/antitoxin systems. An animal model revealed a reduced survival in the lungs and ear by SCV cells. This is the first study documenting the effect of carbon source and the development of a distinct S. pneumoniae cell type during H. influenzae/S. pneumoniae interactions. |
Keywords: | Streptococcus pneumoniae; Haemophilus influenzae; co-infection; small colony variants; bacterial pathogenesis; molecular adaptation; dormant |
Rights: | © FEMS 2018. All rights reserved. |
DOI: | 10.1093/femspd/fty074 |
Grant ID: | http://purl.org/au-research/grants/arc/DE140100963 |
Published version: | http://dx.doi.org/10.1093/femspd/fty074 |
Appears in Collections: | Aurora harvest 8 Environment Institute publications |
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