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https://hdl.handle.net/2440/11804
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Type: | Journal article |
Title: | Impact of restriction of placental and fetal growth on expression of 11b-hydroxysteroid dehydrogenase type 1 and type 2 messenger ribonucleic acid in the liver, kidney, and adrenal of the sheep fetus |
Author: | McMillen, I. Warnes, K. Adams, M. Robinson, J. Owens, J. Coulter, C. |
Citation: | Endocrinology, 2000; 141(2):539-543 |
Publisher: | Endocrine Soc |
Issue Date: | 2000 |
ISSN: | 0013-7227 1945-7170 |
Abstract: | We have investigated the effects of fetal growth restriction, induced by restriction of placental growth and function (PR), on 11beta-hydroxysteroid dehydrogenase type 1 (11betaHSD-1) and 11betaHSD-2 messenger RNA (mRNA) expression in fetal tissues in the sheep, using Northern blot analysis. Fetal liver, kidney, and adrenals were collected from normally grown fetuses at 90 days (n = 6), 125 days (n = 6), and 141-145 days (n = 7) and from PR fetuses at 141-145 days (n = 6). Expression of 11betaHSD-1 mRNA in the fetal liver increased significantly between 125 days (7.4+/-0.8) and 141-145 days gestation (27+/-5.3). There was also an approximately 2-fold increase in the ratio of 11betaHSD-1 mRNA/18S rRNA expression in the PR group (53.8+/-7.9) compared with that in control animals at 141-145 days gestation. There was a significant decrease in 11betaHSD-2 mRNA in fetal adrenals between 125 days (41.6+/-2.4) and 141-145 days (26.7+/-1.1) gestation, but there was no effect of PR on the expression of adrenal 11betaHSD-2 mRNA. 11betaHSD-2 mRNA expression in the fetal kidney increased between 90 days (16.8+/-1.7) and 141-145 days gestation (31.7+/-4.3), but there was no effect of PR on the levels of 11betaHSD-2 mRNA in the fetal kidney. In summary, 11betaHSD-2 mRNA is differentially regulated in the fetal adrenal and kidney in the sheep fetus during late gestation. There is also a specific increase in the expression of 11betaHSD-1 mRNA in the liver of growth-restricted fetuses in late gestation. This suggests that there is increased hepatic exposure to cortisol in the growth-restricted fetus, which may be important in the reprogramming of hepatic physiology that occurs after growth restriction in utero. |
Keywords: | Liver Kidney Adrenal Glands Placenta Animals Sheep Fetal Growth Retardation Body Weight Isoenzymes Hydroxysteroid Dehydrogenases 11-beta-Hydroxysteroid Dehydrogenase Type 1 RNA, Messenger Transcription, Genetic Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Embryonic and Fetal Development Gestational Age Pregnancy Female |
DOI: | 10.1210/endo.141.2.7338 |
Published version: | http://dx.doi.org/10.1210/en.141.2.539 |
Appears in Collections: | Aurora harvest 7 Physiology publications |
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