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https://hdl.handle.net/2440/124961
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Type: | Journal article |
Title: | Phenotypic characterization of osteoarthritic osteocytes from the sclerotic zones: a possible pathological role in subchondral bone sclerosis |
Author: | Jaiprakash, A. Prasadam, I. Feng, J.Q. Liu, Y. Crawford, R. Xiao, Y. |
Citation: | International Journal of Biological Sciences, 2012; 8(3):406-417 |
Publisher: | Ivyspring International Publisher |
Issue Date: | 2012 |
ISSN: | 1449-2288 1449-2288 |
Statement of Responsibility: | Anjali Jaiprakash, Indira Prasadam, Jian Q. Feng, Ying Liu, Ross Crawford, Yin Xiao |
Abstract: | Subchondral bone sclerosis is a well-recognised manifestation of osteoarthritis (OA). The osteocyte cell network is now considered to be central to the regulation of bone homeostasis; however, it is not known whether the integrity of the osteocyte cell network is altered in OA patients. The aim of this study was to investigate OA osteocyte phenotypic changes and its potential role in OA subchondral bone pathogenesis. The morphological and phenotypic changes of osteocytes in OA samples were investigated by micro-CT, SEM, histology, immunohistochemistry, TRAP staining, apoptosis assay and real-time PCR studies. We demonstrated that in OA subchondral bone, the osteocyte morphology was altered showing rough and rounded cell body with fewer and disorganized dendrites compared with the osteocytes in control samples. OA osteocyte also showed dysregulated expression of osteocyte markers, apoptosis, and degradative enzymes, indicating that the phenotypical changes in OA osteocytes were accompanied with OA subchondral bone remodelling (increased osteoblast and osteoclast activity) and increased bone volume with altered mineral content. Significant alteration of osteocytes identified in OA samples indicates a potential regulatory role of osteocytes in subchondral bone remodelling and mineral metabolism during OA pathogenesis. |
Keywords: | Osteocyte; osteoarthritis; subchondral bone; sclerosis; DMP1 and SOST |
Rights: | © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/ licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
DOI: | 10.7150/ijbs.4221 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1032738 |
Published version: | http://dx.doi.org/10.7150/ijbs.4221 |
Appears in Collections: | Aurora harvest 8 Biochemistry publications |
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