Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/124984
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Reduced lymphocyte longevity and homeostatic proliferation in lamin B receptor-deficient mice results in profound and progressive lymphopenia |
Author: | Verhagen, A.M. De Graaf, C.A. Baldwin, T.M. Goradia, A. Collinge, J.E. Kile, B.T. Metcalf, D. Starr, R. Hilton, D.J. |
Citation: | Journal of Immunology, 2012; 188(1):122-134 |
Publisher: | American Association of Immunologists |
Issue Date: | 2012 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Anne M. Verhagen, Carolyn A. de Graaf, Tracey M. Baldwin, Ankita Goradia, Janelle E. Collinge, Benjamin T. Kile ... et al. |
Abstract: | The lamin B receptor (LBR) is a highly unusual inner nuclear membrane protein with multiple functions. Reduced levels are associated with decreased neutrophil lobularity, whereas complete absence of LBR results in severe skeletal dysplasia and in utero/perinatal lethality. We describe a mouse pedigree, Lym3, with normal bone marrow and thymic development but profound and progressive lymphopenia particularly within the T cell compartment. This defect arises from a point mutation within the Lbr gene with only trace mutant protein detectable in homozygotes, albeit sufficient for normal development. Reduced T cell homeostatic proliferative potential and life span in vivo were found to contribute to lymphopenia. To investigate the role of LBR in gene silencing in hematopoietic cells, we examined gene expression in wild-type and mutant lymph node CD8 T cells and bone marrow neutrophils. Although LBR deficiency had a very mild impact on gene expression overall, for common genes differentially expressed in both LBR-deficient CD8 T cells and neutrophils, gene upregulation prevailed, supporting a role for LBR in their suppression. In summary, this study demonstrates that LBR deficiency affects not only nuclear architecture but also proliferation, cell viability, and gene expression of hematopoietic cells. |
Keywords: | Lymphopenia |
Rights: | © 2011 by The American Association of Immunologists |
DOI: | 10.4049/jimmunol.1100942 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/461219 |
Published version: | http://dx.doi.org/10.4049/jimmunol.1100942 |
Appears in Collections: | Aurora harvest 4 Medical Sciences publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.