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https://hdl.handle.net/2440/126977
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Type: | Book chapter |
Title: | Analysis of histone modifications in rodent pancreatic islets by native chromatin immunoprecipitation |
Author: | Sandovici, I. Nicholas, L.M. O’Neill, L.P. |
Citation: | Type 2 Diabetes, 2020 / Stocker, C. (ed./s), vol.2076, Ch.11, pp.199-213 |
Publisher: | Humana Press |
Publisher Place: | New York, NY; U.S.A. |
Issue Date: | 2020 |
Series/Report no.: | Methods In Molecular Biology; 2076 |
ISBN: | 9781493998807 |
Editor: | Stocker, C. |
Statement of Responsibility: | Ionel Sandovici, Lisa M. Nicholas, and Laura P. O'Neill |
Abstract: | The islets of Langerhans are clusters of cells dispersed throughout the pancreas that produce several hormones essential for controlling a variety of metabolic processes, including glucose homeostasis and lipid metabolism. Studying the transcriptional control of pancreatic islet cells has important implications for understanding the mechanisms that control their normal development, as well as the pathogenesis of metabolic diseases such as diabetes. Histones represent the main protein components of the chromatin and undergo diverse covalent modifications that are very important for gene regulation. Here we describe the isolation of pancreatic islets from rodents and subsequently outline the methods used to immunoprecipitate and analyze the native chromatin obtained from these cells. |
Keywords: | Islets of Langerhans; Histone modifications; Nucleosomes; Native chromatin immunoprecipitation (NChIP); Antibodies |
Rights: | © Springer Science+Business Media, LLC, part of Springer Nature 2020 |
DOI: | 10.1007/978-1-4939-9882-1_11 |
Published version: | https://link.springer.com/book/10.1007/978-1-4939-9882-1 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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