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https://hdl.handle.net/2440/128383
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Type: | Journal article |
Title: | A novel method for detecting antigen-specific human regulatory T cells |
Author: | Ebert, L.M. MacRaild, S.E. Davis, I.D. Cebon, J. Chen, W. |
Citation: | Journal of Immunological Methods, 2012; 377(1-2):56-61 |
Publisher: | Elsevier |
Issue Date: | 2012 |
ISSN: | 0022-1759 1872-7905 |
Statement of Responsibility: | Lisa M.Ebert, Sarah E.MacRaild, Ian D.Davis, Jonathan Cebon, Weisan Chen |
Abstract: | Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens. |
Keywords: | Fox P3, Antigen-specific regulatory T cells; CD3 down-regulation; NY-ESO-1 |
Rights: | © 2012 Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.jim.2012.01.004 |
Grant ID: | NHMRC |
Published version: | http://dx.doi.org/10.1016/j.jim.2012.01.004 |
Appears in Collections: | Aurora harvest 8 Biochemistry publications |
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