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https://hdl.handle.net/2440/133791
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Type: | Journal article |
Title: | Podocalyxin inhibits human embryo implantation in vitro and luminal podocalyxin in putative receptive endometrium is associated with implantation failure in fertility treatment |
Author: | Heng, S. Samarajeewa, N. Aberkane, A. Essahib, W. Van de Velde, H. Scelwyn, M. Hull, M.L. Vollenhoven, B. Rombauts, L.J. Nie, G. |
Citation: | Fertility and Sterility, 2021; 116(5):1391-1401 |
Publisher: | Elsevier |
Issue Date: | 2021 |
ISSN: | 0015-0282 1556-5653 |
Statement of Responsibility: | Sophea Heng, Nirukshi Samarajeewa, Asma Aberkane, Wafaa Essahib, Hilde Van de Velde, Maxine Scelwyn, Louise Hull, Beverley Vollenhoven, Luk J. Rombauts, and Guiying Nie |
Abstract: | Objective: To study whether endometrial epithelial podocalyxin (PCX) inhibits implantation of human embryos in vitro and in patients undergoing in vitro fertilization (IVF). Design: We have recently identified PCX as a key negative regulator of endometrial epithelial receptivity. Podocalyxin is expressed in all epithelial cells in the nonreceptive endometrium, but is selectively downregulated in the luminal epithelium (LE) for receptivity. In the current study, we first investigated whether high levels of PCX in Ishikawa monolayer inhibit attachment and/or penetration of human blastocysts in in vitro models. We then examined PCX by immunohistochemistry in putative receptive endometrial tissues biopsied from 81 IVF patients who underwent frozen embryo transfer in the next natural cycle and retrospectively analyzed the association between PCX staining in LE and clinical pregnancy as a proxy of successful implantation. Setting: RMIT University, Australia; Vrije Universiteit Brussel, Belgium. Patient(s): In vitro fertilization patients undergoing frozen/thawed embryo transfer. Intervention(s): N/A. Main Outcome Measure(s): Endometrial epithelial PCX inhibits implantation of human embryos in vitro and in IVF patients. Result(s): High levels of PCX in Ishikawa monolayer significantly inhibited blastocyst attachment and penetration. Among the 81 putative receptive tissues, 73% were negative, but 27% were heterogeneously positive for PCX in LE. The clinical pregnancy rate was 53% in those with a PCX-negative LE but only 18% in those with a PCX-positive LE. If LE was positive for PCX, the odds ratio of no clinical pregnancy was 4.95 (95% Confidence interval, 1.48–14.63). |
Keywords: | Endometrial receptivity IVF Podocalyxin, embryo implantation epithelial receptivity |
Rights: | ©2021 American Society for Reproductive Medicine, Published by Elsevier Inc. This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed. |
DOI: | 10.1016/j.fertnstert.2021.06.028 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1139568 http://purl.org/au-research/grants/nhmrc/1156666 |
Published version: | http://dx.doi.org/10.1016/j.fertnstert.2021.06.028 |
Appears in Collections: | Obstetrics and Gynaecology publications |
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File | Description | Size | Format | |
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hdl_133791.pdf | Published version | 1.12 MB | Adobe PDF | View/Open |
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