Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/134728
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Type: | Journal article |
Title: | Are late hernia mesh complications linked to Staphylococci biofilms? |
Author: | Patiniott, P. Jacombs, A. Kaul, L. Hu, H. Warner, M. Klosterhalfen, B. Karatassas, A. Maddern, G. Richter, K. |
Citation: | Hernia, 2022; 26(5):1293-1299 |
Publisher: | Springer |
Issue Date: | 2022 |
ISSN: | 1265-4906 1248-9204 |
Statement of Responsibility: | P. Patiniott, A. Jacombs, L. Kaul, H. Hu, M. Warner, B. Klosterhalfen, A. Karatassas, G. Maddern, K. Richter |
Abstract: | Purpose: The purpose of this study was to investigate the link between bacterial biofilms and negative outcomes of hernia repair surgery. As biofilms are known to play a role in mesh-related infections, we investigated the presence of biofilms on hernia meshes, which had to be explanted due to mesh failure without showing signs of bacterial infection. Methods: In this retrospective observational study, 20 paraffin-embedded tissue sections from explanted groin hernia meshes were analysed. Meshes have been removed due to chronic pain, hernia recurrence or mesh shrinkage. The presence and bacterial composition of biofilms were determined. First, specimens were stained with fluorescence in situ hybridisation (FISH) probes, specific for Staphylococcus aureus and coagulase-negative staphylococci, and visualised by confocal laser scanning microscopy. Second, DNA was extracted from tissue and identified by S. aureus and S. epidermidis specific PCR. Results: Confocal microscopy showed evidence of bacterial biofilms on meshes in 15/20 (75.0%) samples, of which 3 were positive for S. aureus, 3 for coagulase-negative staphylococci and 9 for both species. PCR analysis identified biofilms in 17/20 (85.0%) samples, of which 4 were positive for S. aureus, 4 for S. epidermidis and 9 for both species. Combined results from FISH/microscopy and PCR identified staphylococci biofilms in 19/20 (95.0%) mesh samples. Only 1 (5.0%) mesh sample was negative for bacterial biofilm by both techniques. Conclusion: Results suggest that staphylococci biofilms may be associated with hernia repair failure. A silent, undetected biofilm infection could contribute to mesh complications, chronic pain and exacerbation of disease. |
Keywords: | Hernia; Biofilms; Implant infection; Mesh failure; Mesh complication; Chronic pain; Staphylococcus aureus |
Description: | Published online: 14 March 2022 |
Rights: | © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
DOI: | 10.1007/s10029-022-02583-0 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/GNT1163634 http://purl.org/au-research/grants/nhmrc/GNT2004036 |
Published version: | http://dx.doi.org/10.1007/s10029-022-02583-0 |
Appears in Collections: | Surgery publications |
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hdl_134728.pdf | Published version | 785.27 kB | Adobe PDF | View/Open |
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