Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/135750
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Type: | Journal article |
Title: | Depletion of oocyte dynamin-related protein 1 shows maternal-effect abnormalities in embryonic development |
Author: | Adhikari, D. Lee, I.-W. Al-Zubaidi, U. Liu, J. Zhang, Q.-H. Yuen, W.S. He, L. Winstanley, Y. Sesaki, H. Mann, J.R. Robker, R.L. Carroll, J. |
Citation: | Science Advances, 2022; 8(24):eabl8070-1-eabl8070-15 |
Publisher: | American Association for the Advancement of Science (AAAS) |
Issue Date: | 2022 |
ISSN: | 2375-2548 2375-2548 |
Statement of Responsibility: | Deepak Adhikari, In-won Lee, Usama Al-Zubaidi, Jun Liu, Qing-Hua Zhang, Wai Shan Yuen, Likun He, Yasmyn Winstanley, Hiromi Sesaki, Jeffrey R. Mann, Rebecca L. Robker, John Carroll |
Abstract: | Eggs contain about 200,000 mitochondria that generate adenosine triphosphate and metabolites essential for oocyte development. Mitochondria also integrate metabolism and transcription via metabolites that regulate epigenetic modifiers, but there is no direct evidence linking oocyte mitochondrial function to the maternal epigenome and subsequent embryo development. Here, we have disrupted oocyte mitochondrial function via deletion of the mitochondrial fission factor Drp1. Fission-deficient oocytes exhibit a high frequency of failure in peri- and postimplantation development. This is associated with altered mitochondrial function, changes in the oocyte transcriptome and proteome, altered subcortical maternal complex, and a decrease in oocyte DNA methyla-tion and H3K27me3. Transplanting pronuclei of fertilized Drp1 knockout oocytes to normal ooplasm fails to rescue embryonic lethality. We conclude that mitochondrial function plays a role in establishing the maternal epigenome, with serious consequences for embryo development. |
Keywords: | Oocytes Cytoplasm Mitochondria Humans Dynamins Embryonic Development Pregnancy Female |
Rights: | Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). |
DOI: | 10.1126/sciadv.abl8070 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1165627 http://purl.org/au-research/grants/nhmrc/1112766 http://purl.org/au-research/grants/nhmrc/1117975 |
Published version: | http://dx.doi.org/10.1126/sciadv.abl8070 |
Appears in Collections: | Obstetrics and Gynaecology publications |
Files in This Item:
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hdl_135750.pdf | Published version | 4.51 MB | Adobe PDF | View/Open |
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