Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/139747
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Molecular characterization and pathogenicity evaluation of enterovirus G isolated from diarrheic piglets |
Author: | Ibrahim, Y.M. Zhang, W. Wang, X. Werid, G.M. Fu, L. Yu, H. Wang, Y. |
Citation: | Microbiology Spectrum, 2023; 11(6) |
Publisher: | American Society for Microbiology |
Issue Date: | 2023 |
ISSN: | 2165-0497 2165-0497 |
Editor: | He, B. |
Statement of Responsibility: | Yassein M. Ibrahim, Wenli Zhang, Xinrong Wang, Gebremeskel Mamu Werid, Lizhi Fu, Haidong Yu, Yue Wang |
Abstract: | Four enterovirus G (EV-G) strains were isolated from diarrheic piglets that were negative for common swine enteric viruses. The spherical enterovirus particles of roughly 30-nm diameter were observed under transmission electron microscopy by using plaque-purified enterovirus. The complete genome sequence analysis revealed that each of four enteroviruses contained a papain-like cysteine protease (PLCP) gene between the 2C and 3A junction regions of the viral genome. This insertion encoded a predicted protease similar to the PLCP of porcine torovirus. The phylogenetic analysis based on complete genome with and without PLCP gene revealed that the four isolated EV-G strains were grouped together with global enterovirus G1-PLCP strains, and more closely related to EV-G/PLCP strains previously detected in China, Japan, and Korea (90.3%–92.2% similarities based on nucleotides). The cell susceptibility test demonstrated that the isolated EV-G could infect and replicate in cell lines from various host species. Furthermore, pathogenicity evaluation showed that the isolated EV-Gs induced mild diarrhea, pyrexia, and reduced body weight in infected piglets. The epidemiological investigation revealed a high prevalence of EV-G in swine herds. Together, our findings demonstrate that the isolated EV-G is pathogenic in piglets and may be advantageous in providing more trustworthy data on the evolution and pathological properties of EV-G. |
Keywords: | enterovirus G; characterization; genetic recombination; pathogenicity |
Description: | Published 13 October 2023 |
Rights: | Copyright © 2023 Ibrahim et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
DOI: | 10.1128/spectrum.02643-23 |
Published version: | http://dx.doi.org/10.1128/spectrum.02643-23 |
Appears in Collections: | Animal and Veterinary Sciences publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_139747.pdf | Published version | 3.41 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.