Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/139886
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ahmed, J. | - |
dc.contributor.author | Ismail, A. | - |
dc.contributor.author | Ding, L. | - |
dc.contributor.author | Yool, A.J. | - |
dc.contributor.author | Chaumont, F. | - |
dc.date.issued | 2024 | - |
dc.identifier.citation | Plant, Cell and Environment, 2024; 47(2):527-539 | - |
dc.identifier.issn | 0140-7791 | - |
dc.identifier.issn | 1365-3040 | - |
dc.identifier.uri | https://hdl.handle.net/2440/139886 | - |
dc.description | First published: 09 November 2023 | - |
dc.description.abstract | Plant aquaporins (AQPs) facilitate the membrane diffusion of water and small solutes, including hydrogen peroxide (H2 O2 ) and, possibly, cations, essential signalling molecules in many physiological processes. While the determination of the channel activity generally depends on heterologous expression of AQPs in Xenopus oocytes or yeast cells, we established a genetic tool to determine whether they facilitate the diffusion of H2 O2 through the plasma membrane in living plant cells. We designed genetic constructs to co-express the fluorescent H2 O2 sensor HyPer and AQPs, with expression controlled by a heat shock-inducible promoter in Nicotiana tabacum BY-2 suspension cells. After induction of ZmPIP2;5 AQP expression, a HyPer signal was recorded when the cells were incubated with H2 O2 , suggesting that ZmPIP2;5 facilitates H2 O2 transmembrane diffusion; in contrast, the ZmPIP2;5W85A mutated protein was inactive as a water or H2 O2 channel. ZmPIP2;1, ZmPIP2;4 and AtPIP2;1 also facilitated H2 O2 diffusion. Incubation with abscisic acid and the elicitor flg22 peptide induced the intracellular H2 O2 accumulation in BY-2 cells expressing ZmPIP2;5. We also monitored cation channel activity of ZmPIP2;5 using a novel fluorescent photo-switchable Li+ sensor in BY-2 cells. BY-2 suspension cells engineered for inducible expression of AQPs as well as HyPer expression and the use of Li+ sensors constitute a powerful toolkit for evaluating the transport activity and the molecular determinants of PIPs in living plant cells. | - |
dc.description.statementofresponsibility | Jahed Ahmed, Ahmed Ismail, Lei Ding, Andrea J. Yool, François Chaumont | - |
dc.language.iso | en | - |
dc.publisher | Wiley | - |
dc.rights | © 2023 John Wiley & Sons Ltd. | - |
dc.source.uri | http://dx.doi.org/10.1111/pce.14763 | - |
dc.subject | HyPer biosensor | - |
dc.subject | Li+ sensor | - |
dc.subject | live cell imaging | - |
dc.subject | plasma membrane intrinsic protein (PIP) | - |
dc.subject | tobacco BY-2 suspension cells | - |
dc.title | A new method to measure aquaporin-facilitated membrane diffusion of hydrogen peroxide and cations in plant suspension cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1111/pce.14763 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP190101745 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Yool, A.J. [0000-0003-1283-585X] | - |
Appears in Collections: | Physiology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.