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https://hdl.handle.net/2440/140751
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Type: | Journal article |
Title: | Viewing early life without labels: optical approaches for imaging the early embryo |
Author: | Chow, D.J.X. Tan, T.C.Y. Upadhya, A. Lim, M. Dholakia, K. Dunning, K.R. |
Citation: | Biology of Reproduction, 2024; 110(6):ioae062-1-ioae062-18 |
Publisher: | Oxford University Press |
Issue Date: | 2024 |
ISSN: | 0006-3363 0006-3363 |
Statement of Responsibility: | Darren J.X. Chow, Tiffany C.Y. Tan, Avinash Upadhya, Megan Lim, Kishan Dholakia, and Kylie R. Dunning |
Abstract: | Embryo quality is an important determinant of successful implantation and a resultant live birth. Current clinical approaches for evaluating embryo quality rely on subjective morphology assessments or an invasive biopsy for genetic testing. However, both approaches can be inherently inaccurate and crucially, fail to improve the live birth rate following the transfer of in vitro produced embryos. Optical imaging offers a potential non-invasive and accurate avenue for assessing embryo viability. Recent advances in various label-free optical imaging approaches have garnered increased interest in the field of reproductive biology due to their ability to rapidly capture images at high resolution, delivering both morphological and molecular information. This burgeoning field holds immense potential for further development, with profound implications for clinical translation. Here, our review aims to: (1) describe the principles of various imaging systems, distinguishing between approaches that capture morphological and molecular information, (2) highlight the recent application of these technologies in the field of reproductive biology, and (3) assess their respective merits and limitations concerning the capacity to evaluate embryo quality. Additionally, the review summarizes challenges in the translation of optical imaging systems into routine clinical practice, providing recommendations for their future development. Finally, we identify suitable imaging approaches for interrogating the mechanisms underpinning successful embryo development. |
Keywords: | autofluorescence embryo assessment fluorescent lifetime microscopy hyperspectral imaging label-free imaging light sheet microscopy metabolic imaging non-invasive optical imaging |
Description: | OnlinePubl |
Rights: | © The Author(s) 2024. Published by Oxford University Press on behalf of Society for the Study of Reproduction. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/lice nses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
DOI: | 10.1093/biolre/ioae062 |
Grant ID: | http://purl.org/au-research/grants/arc/FL210100099 http://purl.org/au-research/grants/nhmrc/2003786 http://purl.org/au-research/grants/nhmrc/2029067 |
Published version: | http://dx.doi.org/10.1093/biolre/ioae062 |
Appears in Collections: | Research Outputs |
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hdl_140751.pdf | Published version | 1.92 MB | Adobe PDF | View/Open |
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