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https://hdl.handle.net/2440/14460
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Type: | Journal article |
Title: | Biosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-β-acylglucuronide |
Other Titles: | Biosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-beta-acylglucuronide |
Author: | Sallustio, B. Fairchild, B. |
Citation: | Journal of Chromatography A, 1995; 665(2):345-353 |
Publisher: | Elsevier |
Issue Date: | 1995 |
ISSN: | 0021-9673 1879-1794 |
Statement of Responsibility: | Benedetta C. Sallustio, Barbara A. Fairchild |
Abstract: | Gemfibrozil 1-O-β-acylglucuronide was purified from the urine of a volunteer administered gemfibrozil, and an isocratic reversed-phase HPLC method was developed for its direct measurement. Quantitation of gemfibrozil and gemfibrozil 1-O-β-acylglucuronide was carried out from plasma, following extraction from acidified specimens into ethyl acetate, on a 5-μm CN reversed-phase column with a mobile phase (pH 3.5) containing acetonitrile, tetrabutylammonium sulphate and distilled water, using fluorescence detection at 284 nm excitation and 316 nm emission. Calibration curves were linear for both compounds over a concentration range of 0.1 to 40 mg/l, with intra-assay coefficients of variation <5% at concentrations of 20.0, 2.0 and 0.2 mg/l, and inter-assay coefficients of variation <10%. No degradation of gemfibrozil 1-O-β-acylglucuronide was detected as a result of the analytical procedure. However, a preliminary application of the method indicates that gemfibrozil acylglucuronide is chemically unstable undergoing intra-molecular rearrangement and hydrolysis under physiological conditions. |
Keywords: | Humans Gemfibrozil Glucuronates Chromatography, High Pressure Liquid Chromatography, Thin Layer Magnetic Resonance Spectroscopy Drug Stability Hydrogen-Ion Concentration Quality Control Mass Spectrometry |
Rights: | Copyright © 1995 Published by Elsevier B.V. |
DOI: | 10.1016/0378-4347(94)00530-I |
Published version: | http://dx.doi.org/10.1016/0378-4347(94)00530-i |
Appears in Collections: | Aurora harvest 2 Pharmacology publications |
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