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https://hdl.handle.net/2440/23789
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Type: | Journal article |
Title: | The effects of stubble retention and nitrogen application on soil microbial community structure and functional gene abundance under irrigated maize |
Author: | Wakelin, S. Colloff, M. Harvey, P. Marschner, P. Gregg, A. Rogers, S. |
Citation: | FEMS Microbiology Ecology, 2007; 59(3):661-670 |
Publisher: | Elsevier Science BV |
Issue Date: | 2007 |
ISSN: | 0168-6496 1574-6941 |
Statement of Responsibility: | Steven A. Wakelin, Matt J. Colloff, Paul R. Harvey, Petra Marschner, Adrienne L. Gregg & Stephen L. Rogers |
Abstract: | The effects of agronomic management practices on the soil microbial community were investigated in a maize production system in New South Wales, Australia. The site has been intensively studied to measure the impact of stubble management and N-fertilizer application on greenhouse gas emissions (CO₂ and N₂O), N-cycling, pathology, soil structure and yield. As all of these endpoints can be regulated by microbial processes, the microbiology of the system was examined. Soil samples were taken after a winter fallow period and the diversity of the bacterial and fungal communities was measured using PCR-denaturing gradient gel electrophoresis. Stubble and N shifted the structure of bacterial and fungal communities with the primary driver being stubble addition on the fungal community structure (P<0.05 for all effects). Changes in C, N (total and NO3), K and Na, were correlated (P<0.05) with variation in the microbial community structure. Quantitative PCR showed that nifH (nitrogen fixation) and napA (denitrification) gene abundance increased upon stubble retention, whereas amoA gene numbers were increased by N addition. These results showed that the management of both stubble and N have significant and long-term impacts on the size and structure of the soil microbial community at phylogenetic and functional levels. |
Keywords: | Bacteria Fungi Zea mays Nitrogen Soil Fertilizers Electrophoresis, Gel, Two-Dimensional Cluster Analysis Polymerase Chain Reaction Soil Microbiology Nitrogen Fixation Genes, Bacterial Genes, Fungal Agriculture Australia |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1111/j.1574-6941.2006.00235.x |
Published version: | http://dx.doi.org/10.1111/j.1574-6941.2006.00235.x |
Appears in Collections: | Aurora harvest 6 Earth and Environmental Sciences publications |
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