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https://hdl.handle.net/2440/27247
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Type: | Journal article |
Title: | Differentiation of polyphosphate metabolism between the extra- and intraradical hyphae of arbuscular mycorrhizal fungi |
Author: | Ezawa, T. Smith, S. Smith, F. |
Citation: | New Phytologist, 2001; 149(3):555-563 |
Publisher: | Cambridge Univ Press |
Issue Date: | 2001 |
ISSN: | 0028-646X 1469-8137 |
Statement of Responsibility: | Tatsuhiro, Ezawa; Sally E., Smith; F., Andrew Smith |
Abstract: | • Regulation of polyphosphate metabolism is reported in arbuscular mycorrhizal symbiosis. • Marigold (Tagetes patula) plants inoculated with Glomus coronatum or Glomus etunicatum were grown in mesh bags. Exopolyphosphatase activity in extra- and intraradical hyphae was measured and characterized. The hyphae were stained with Neutral red to show acidic vacuoles in which polyphosphate synthesis might occur. • Exopolyphosphate activity was differently expressed between the extra- and intraradical hyphae, as indicated by different pH optima; high activity was observed at pH 5.0 in the intraradical hyphae of both fungal species. K<sub>m</sub> values were lower at neutral pH with long-chain polyphosphate, whereas acidic activity showed lower K<sub>m</sub> with short-chain polyphosphate. Both extra- and intradical hyphae had acidic vacuoles. Polyphosphate occurred in the hyphae of the high-P, but not the low-P treatment. By contrast, exopolyphosphatase activity and vacuolar acidity were relatively constant irrespective of polyphosphate status. • The fungi have at least two different exopolyphosphatase-type enzymes which are differently expressed between extra- and intraradical hyphae; polyphosphate accumulation might be a dynamic balance between synthesis and hydrolysis. |
Keywords: | Neutral red acid phosphatase acidic vacuole arbuscular mycorrhizal fungi exopolyphosphatase polyphosphate |
DOI: | 10.1046/j.1469-8137.2001.00040.x |
Published version: | http://dx.doi.org/10.1046/j.1469-8137.2001.00040.x |
Appears in Collections: | Aurora harvest 2 Soil and Land Systems publications |
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