Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/27433
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Visualization of mycorrhizal fungal structures in resin embedded tissues with xanthene dyes using laser scanning confocal microscopy |
Author: | Melville, L. Dickson, S. Farquhar, M. Smith, S. Peterson, R. |
Citation: | Botany, 1998; 76(1):174-178 |
Publisher: | NATL RESEARCH COUNCIL CANADA |
Issue Date: | 1998 |
ISSN: | 0008-4026 |
Statement of Responsibility: | Melville, Lewis Dickson, Sandy ; Farquhar, Melissa L ; Smith, S E ; Peterson, R. Larry |
Abstract: | <jats:p>The xanthene dyes sulforhordamine G, phloxine B, rose Bengal, and 4,5,6,7-tetrachloroflorescein were used as fluorochromes for laser scanning confocal microscopy of LR-White resin-embedded mycorrhizae. Sulforhodamine G was the most effective dye, giving an even staining of cell components throughout the material, with minimal background fluorescence of LR-White resin. Confocal microscopy of stained blocks of tissue on a slide, viewed without the use of a coverslip, revealed the three-dimensional nature of various mycorrhizal structures; these structures included arbuscules, vesicles, and coiled hyphae in arbuscular mycorrhizae; coiled hyphae in orchid mycorrhizae; mantle and Hartig net hyphae in ectomycorrhizae; and intracellular hyphae in arbutoid mycorrhizae. Sections mounted on slides viewed with confocal microscopy provided exceptional clarity of fungal form and cytoplasmic contents and showed the relationship to the plant cells, also with negligible background fluorescence. Mounting and staining blocks of resin-embedded material provided a fast and effective technique for the visualization of a variety of plant and fungal tissues. Stain penetration in whole-mounted samples was sufficient to reconstruct clear three-dimensional images using confocal microscopy.Key words: mycorrhizae, xanthene dyes, confocal microscopy, resin embedding.</jats:p> |
DOI: | 10.1139/b97-158 |
Published version: | http://dx.doi.org/10.1139/b97-158 |
Appears in Collections: | Aurora harvest 6 Soil and Land Systems publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.