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https://hdl.handle.net/2440/27561
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Type: | Journal article |
Title: | Constitutive regulation of the Insl3 gene in rat Leydig cells |
Author: | Sadeghain, H. Anand Ivell, R. Balvers, M. Relan, V. Ivell, R. |
Citation: | Molecular and Cellular Endocrinology, 2005; 241(1-2):10-20 |
Publisher: | Elsevier Sci Ireland Ltd |
Issue Date: | 2005 |
ISSN: | 0303-7207 1872-8057 |
Statement of Responsibility: | Helen Sadeghian, Ravinder Anand-Ivell, Marga Balvers, Vandana Relan and Richard Ivell |
Abstract: | Insulin-like factor 3 (Insl3) is a major new product of the Leydig cells in all mammalian species so far examined. The rat Insl3 gene is encoded by two exons in close juxtaposition to the Jak3 gene. Using RT-PCR analysis we now show that in the rat testis it is expressed as both major and minor splice variants, the former encoding the normal protein, the latter a truncated peptide comprising a C-terminally extended B-domain. Both transcripts are produced in constant relative amounts uniquely in the Leydig cells of the postnatal testis and in no other testicular cell type. Rat Insl3 protein is also expressed only in Leydig cells after postnatal day 30. Although specific mRNA is present at earlier times, corresponding protein is not detected. Semi-quantitative RT-PCR analysis of Insl3 transcripts in the mouse MA-10 tumour Leydig cell-line under a wide range of stimulation regimes shows that in an acute context, the Insl3 gene is expressed absolutely constitutively. This is confirmed by transfection and electrophoretic mobility shift (EMSA) analysis of the rat Insl3 gene promoter, wherein the importance of three putative SF-1 responsive elements is underscored, although these appear to differ in their relative importance from their counterparts in the mouse Insl3 gene. |
Keywords: | Leydig Cells Cell Line, Tumor Animals Mice Rats Rats, Wistar Insulin Proteins Homeodomain Proteins Receptors, Cytoplasmic and Nuclear Transcription Factors RNA, Messenger Gene Expression Regulation Response Elements Male Protein-Tyrosine Kinases Janus Kinase 3 Steroidogenic Factor 1 |
Description: | Copyright © 2005 Elsevier Ireland Ltd All rights reserved. |
DOI: | 10.1016/j.mce.2005.03.017 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/506028/description#description |
Published version: | http://dx.doi.org/10.1016/j.mce.2005.03.017 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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