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https://hdl.handle.net/2440/27575
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Type: | Journal article |
Title: | Additive inhibition of complement deposition by pneumolysin and PspA facilitates Streptococcus pneumoniae septicemia |
Author: | Yuste, J. Botto, M. Paton, J. Holden, D. Brown, J. |
Citation: | Journal of Immunology, 2005; 175(3):1813-1819 |
Publisher: | Amer Assoc Immunologists |
Issue Date: | 2005 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Jose Yuste, Marina Botto, James C. Paton, David W. Holden and Jeremy S. Brown |
Abstract: | Streptococcus pneumoniae is a common cause of septicemia in the immunocompetent host. To establish infection, S. pneumoniae has to overcome host innate immune responses, one component of which is the complement system. Using isogenic bacterial mutant strains and complement-deficient immune naive mice, we show that the S. pneumoniae virulence factor pneumolysin prevents complement deposition on S. pneumoniae, mainly through effects on the classical pathway. In addition, using a double pspA–/ply– mutant strain we demonstrate that pneumolysin and the S. pneumoniae surface protein PspA act in concert to affect both classical and alternative complement pathway activity. As a result, the virulence of the pspA–/ply– strain in models of both systemic and pulmonary infection is greatly attenuated in wild-type mice but not complement deficient mice. The sensitivity of the pspA–/ply– strain to complement was exploited to demonstrate that although early innate immunity to S. pneumoniae during pulmonary infection is partially complement-dependent, the main effect of complement is to prevent spread of S. pneumoniae from the lungs to the blood. These data suggest that inhibition of complement deposition on S. pneumoniae by pneumolysin and PspA is essential for S. pneumoniae to successfully cause septicemia. Targeting mechanisms of complement inhibition could be an effective therapeutic strategy for patients with septicemia due to S. pneumoniae or other bacterial pathogens. |
Keywords: | Animals Mice, Inbred C57BL Mice, Knockout Mice Streptococcus pneumoniae Pneumococcal Infections Sepsis Complement Factor B Bacterial Proteins Heat-Shock Proteins Streptolysins Virulence Factors Complement Pathway, Classical Drug Synergism Complement System Proteins Complement Inactivator Proteins Complement C3 Complement C1q |
Rights: | © 2005 by The American Association of Immunologists |
DOI: | 10.4049/jimmunol.175.3.1813 |
Published version: | http://www.jimmunol.org/cgi/content/abstract/175/3/1813 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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