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https://hdl.handle.net/2440/27592
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Type: | Journal article |
Title: | Pre-treatment with insulin-like growth factor-I partially ameliorates 5-fluorouracil-induced intestinal mucositis in rats |
Author: | Cool, J. Wright, J. Xian, C. Butler, R. Geier, M. Howarth, G. |
Citation: | Growth Hormone and IGF Research, 2005; 15(1):72-82 |
Publisher: | Churchill Livingstone |
Issue Date: | 2005 |
ISSN: | 1096-6374 1532-2238 |
Statement of Responsibility: | Johanna C. Cool, Jodie L. Dyer, Cory J. Xian, Ross N. Butler, Mark S. Geier and Gordon S. Howarth |
Abstract: | Insulin-like growth factor-I (IGF-I) has been demonstrated to enhance mucosal repair following intestinal damage induced by chemotherapeutic agents (intestinal mucositis). However, the potential for prophylactic IGF-I to protect the intestine remains undefined. We investigated the effects of IGF-I pre-treatment on chemotherapy-induced mucositis in rats. Male Sprague Dawley rats were treated for 7 days with 0 or 4.3 mg/kg/day IGF-I delivered systemically via osmotic mini-pump. Rats received an intraperitoneal injection of 0 or 150 mg/kg 5-fluorouracil (5-FU) on day 7 and were killed 48 h later for assessment of intestinal damage and repair. Compared to normal controls, 5-FU decreased epithelial proliferation by 86%, concurrently increasing the incidence of apoptosis 87-fold, whilst decreasing small intestinal (SI) length by 14%, SI weight by 30% and total gut weight by 24%. 5-FU decreased villus height in the duodenum (23%), jejunum (20%) and ileum (30%) with crypt depths decreased by 31%, 27% and 33% in these gut regions. These effects were less profound in IGF-I pre-treated rats in which apoptosis was increased 48-fold, with SI length decreased by 7%, SI weight by 18% and total gut weight by 15% accompanied by decreases in villus height of 8% (duodenum), 14% (jejunum) and 21% (ileum), and crypt depth decreases of 23%, 16% and 17% for the same gut regions, compared to normal controls. We conclude that IGF-I pre-treatment only partially attenuates features of intestinal mucositis when assessed 48 h after 5-FU chemotherapy. |
Keywords: | Insulin-like growth factor-I Mucosa Chemotherapy Intestinal injury Rats |
DOI: | 10.1016/j.ghir.2004.12.002 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/623041/description#description |
Published version: | http://dx.doi.org/10.1016/j.ghir.2004.12.002 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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