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https://hdl.handle.net/2440/27607
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Type: | Journal article |
Title: | A family of RS domain proteins with novel subcellular localization and trafficking |
Author: | Kavanagh, S. Schulz, T. Davey, P. Claudianos, C. Russell, C. Rathjen, P. |
Citation: | Nucleic Acids Research, 2005; 33(4):1309-1322 |
Publisher: | Oxford Univ Press |
Issue Date: | 2005 |
ISSN: | 0305-1048 1362-4962 |
Organisation: | Centre for the Molecular Genetics of Development |
Abstract: | We report the sequence, conservation and cell biology of a novel protein, Psc1, which is expressed and regulated within the embryonic pluripotent cell population of the mouse. The Psc1 sequence includes an RS domain and an RNA recognition motif (RRM), and a sequential arrangement of protein motifs that has not been demonstrated for other RS domain proteins. This arrangement was conserved in a second mouse protein (BAC34721. The identification of Psc1 and BAC34721homologues in vertebrates and related proteins, more widely throughout evolution, defines a new family of RS domain proteins termed acidic rich RS (ARRS) domain proteins. Psc1 incorporated into the nuclear speckles, but demonstrated novel aspects of subcellular distribution including localization to speckles proximal to the nuclear periphery and localization to punctate structures in the cytoplasm termed cytospeckles. Integration of Psc1 into cytospeckles was dependent on the RRM. Cytospeckles were dynamic within the cytoplasm and appeared to traffic into the nucleus. These observations suggest a novel role in RNA metabolism for ARRS proteins. |
Keywords: | COS Cells Cell Nucleus Cell Nucleus Structures Cytoplasm Cytoplasmic Structures Microtubules Animals Mice Proteins RNA-Binding Proteins Nuclear Proteins DNA, Complementary Evolution, Molecular Binding Sites Amino Acid Sequence Conserved Sequence Protein Structure, Tertiary Active Transport, Cell Nucleus Molecular Sequence Data Chlorocebus aethiops |
DOI: | 10.1093/nar/gki269 |
Published version: | http://nar.oxfordjournals.org/cgi/content/abstract/33/4/1309 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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