Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/27613
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Type: Journal article
Title: Identification and characterization of avihepadnaviruses isolated from exotic anseriformes maintained in captivity
Author: Guo, H.
Mason, W.
Aldrich, C.
Saputelli, J.
Miller, D.
Jilbert, A.
Newbold, J.
Citation: Journal of Virology, 2005; 79(5):2729-2742
Publisher: Amer Soc Microbiology
Issue Date: 2005
ISSN: 0022-538X
1098-5514
Statement of
Responsibility: 
Haitao Guo, William S. Mason, Carol E. Aldrich, Jeffry R. Saputelli, Darren S. Miller, Allison R. Jilbert and John E. Newbold
Abstract: Five new hepadnaviruses were cloned from exotic ducks and geese, including the Chiloe wigeon, mandarin duck, puna teal, Orinoco sheldgoose, and ashy-headed sheldgoose. Sequence comparisons revealed that all but the mandarin duck viruses were closely related to existing isolates of duck hepatitis B virus (DHBV), while mandarin duck virus clones were closely related to Ross goose hepatitis B virus. Nonetheless, the S protein, core protein, and functional domains of the Pol protein were highly conserved in all of the new isolates. The Chiloe wigeon and puna teal hepatitis B viruses, the two new isolates most closely related to DHBV, also lacked an AUG start codon at the beginning of their X open reading frame (ORF). But as previously reported for the heron, Ross goose, and stork hepatitis B viruses, an AUG codon was found near the beginning of the X ORF of the mandarin duck, Orinoco, and ashy-headed sheldgoose viruses. In all of the new isolates, the X ORF ended with a stop codon at the same position. All of the cloned viruses replicated when transfected into the LMH line of chicken hepatoma cells. Significant differences between the new isolates and between these and previously reported isolates were detected in the pre-S domain of the viral envelope protein, which is believed to determine viral host range. Despite this, all of the new isolates were infectious for primary cultures of Pekin duck hepatocytes, and infectivity in young Pekin ducks was demonstrated for all but the ashy-headed sheldgoose isolate.
Keywords: Cell Line
Animals
Animals, Domestic
Anseriformes
Ducks
Geese
Chickens
Avihepadnavirus
Viral Proteins
DNA, Viral
In Situ Hybridization
Phylogeny
Virus Replication
Virulence
Species Specificity
Amino Acid Sequence
Base Sequence
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Molecular Sequence Data
Female
Male
DOI: 10.1128/JVI.79.5.2729-2742.2005
Published version: http://jvi.asm.org/cgi/content/abstract/79/5/2729
Appears in Collections:Aurora harvest 6
Molecular and Biomedical Science publications

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