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https://hdl.handle.net/2440/28092
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Type: | Journal article |
Title: | Kinetics of human immunodeficiency virus type 1 (HIV) DNA integration in acutely infected cells as determined using a novel assay for detection of integrated HIV DNA |
Author: | Vandegraaff, N. Kumar, R. Burrell, C. Li, P. |
Citation: | Journal of Virology, 2001; 75(22):11253-11260 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2001 |
ISSN: | 0022-538X 1098-5514 |
Statement of Responsibility: | Nick Vandegraaff, Raman Kumar, Christopher J. Burrell, and Peng Li |
Abstract: | We have developed a novel linker-primer PCR assay for the detection and quantification of integrated human immunodeficiency virus type 1 (HIV) DNA. This assay reproducibly allowed the detection of 10 copies of integrated HIV DNA, in a background of 2 × 105 cell equivalents of human chromosomal DNA, without amplifying extrachromosomal HIV DNA. We have used this assay and a near-synchronous one-step T-cell infection model to investigate the kinetics of viral DNA accumulation following HIV infection. We report here that integrated HIV DNA started accumulating 1 h after the first appearance of extrachromosomal viral DNA and accounted for ~10% of the total HIV DNA synthesized in the first round of viral replication. These results highlight the efficient nature of integrase-mediated HIV integration in infected T cells. |
Keywords: | T-Lymphocytes Cell Line Humans HIV-1 DNA, Viral Polymerase Chain Reaction Virus Integration Virus Replication Kinetics |
Description: | Copyright © 2001, American Society for Microbiology |
DOI: | 10.1128/JVI.75.22.11253-11260.2001 |
Published version: | http://jvi.asm.org/cgi/content/abstract/75/22/11253 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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