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https://hdl.handle.net/2440/28098
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Type: | Journal article |
Title: | RcoA has pleiotropic effects on aspergillus nidulans cellular development |
Author: | Hicks, J. Lockington, R. Strauss, J. Dieringer, D. Kubicek, C. Kelly, J. Keller, N. |
Citation: | Molecular Microbiology, 2001; 39(6):1482-1493 |
Publisher: | Blackwell Science Ltd |
Issue Date: | 2001 |
ISSN: | 0950-382X 1365-2958 |
Statement of Responsibility: | Julie Hicks, Robin A. Lockington, Joseph Strauss, Daniel Dieringer, Christian P. Kubicek, Joan Kelly and Nancy Keller |
Abstract: | Aspergillus nidulans rcoA encodes a member of the WD repeat family of proteins. The RcoA protein shares sequence similarity with other members of this protein family, including the Saccharomyces cerevisiae Tup1p and Neurospora crassa RCO1. Tup1p is involved in negative regulation of an array of functions including carbon catabolite repression. RCO1 functions in regulating pleiotropic developmental processes, but not carbon catabolite repression. In A. nidulans, deletion of rcoA (DrcoA), a recessive mutation, resulted in gross defects in vegetative growth, asexual spore production and sterigmatocystin (ST) biosynthesis. Expression of the asexual and ST pathway-specific regulatory genes, brlA and aflR, respectively, but not the signal transduction genes (i.e. flbA, fluG or fadA) regulating brlA and aflR expression was delayed (brlA) or eliminated (aflR) in a DrcoA strain. Overexpression of aflR in a DrcoA strain could not rescue normal expression of downstream targets of AflR. CreAdependent carbon catabolite repression of starch and ethanol utilization was only weakly affected in a DrcoA strain. The strong role of RcoA in development, vegetative growth and ST production, compared with a relatively weak role in carbon catabolite repression, is similar to the role of RCO1 in N. crassa. |
Keywords: | Aspergillus nidulans Carbon Sterigmatocystin GTP-Binding Proteins DNA-Binding Proteins Fungal Proteins Transcription Factors Repressor Proteins Cloning, Molecular Signal Transduction Gene Expression Regulation, Fungal Gene Deletion Amino Acid Sequence Repetitive Sequences, Amino Acid Sequence Homology, Amino Acid Molecular Sequence Data |
Description: | The definitive version is available at www.blackwell-synergy.com |
DOI: | 10.1046/j.1365-2958.2001.02332.x |
Published version: | http://dx.doi.org/10.1046/j.1365-2958.2001.02332.x |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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