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https://hdl.handle.net/2440/28120
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Type: | Journal article |
Title: | The genomic complexity of micro B chromosomes of Brachycome dichromosomatica |
Author: | Houben, A. Verlin, D. Leach, C. Timmis, J. |
Citation: | Chromosoma: biology of the nucleus, 2001; 110(7):451-459 |
Publisher: | Springer-Verlag |
Issue Date: | 2001 |
ISSN: | 0009-5915 1432-0886 |
Statement of Responsibility: | Andreas Houben, Dawn Verlin, Carolyn R. Leach and Jeremy N. Timmis |
Abstract: | A major sequence component of the micro B chromosome of Brachycome dichromosomatica (2n=4) is the tandem repeat Bdm29, which was found by in situ hybridisation to be distributed along the entire length of the chromosome. A high copy number of this sequence does not occur as a regular feature of the A chromosomes in this species but it was found in infrequent individuals in two wild populations that were analysed. In these instances Bdm29 is localised within heterochromatic, polymorphic segments on the long arm of chromosome 1. The origin of the micro B chromosomes was investigated by determining whether they are related to this A chromosome polymorphism by simple excision and/or integration. Results obtained by using Bdm29, together with a newly isolated repeat sequence, Bdm54, and a number of other sequences known to occur on the micro B chromosome, as probes in in situ hybridisation and Southern analysis demonstrated that the formation of micro B chromosomes is a complex multistep process. The observation that the genomic organisation of the micro B chromosome is unlike anything found on the A chromosomes precludes their origin by simple excision and also indicates that micro Bs do not integrate directly into the A complement to form polymorphic heterochomatic segments. |
Keywords: | Chromosomes Cell Nucleus Heterochromatin Telomere Asteraceae Plant Leaves DNA, Plant In Situ Hybridization, Fluorescence Karyotyping Sequence Analysis, DNA Metaphase Base Sequence Repetitive Sequences, Nucleic Acid Genotype Diploidy Polymorphism, Genetic Genome, Plant Molecular Sequence Data |
Description: | The original publication can be found at www.springerlink.com |
DOI: | 10.1007/s00412-001-0173-1 |
Published version: | http://dx.doi.org/10.1007/s00412-001-0173-1 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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