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https://hdl.handle.net/2440/28134
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dc.contributor.author | Denton, D. | - |
dc.contributor.author | Secombe, J. | - |
dc.contributor.author | Coombe, M. | - |
dc.contributor.author | Brumby, A. | - |
dc.contributor.author | Saint, R. | - |
dc.contributor.author | Richardson, H. | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Developmental Biology, 2002; 241(1):157-171 | - |
dc.identifier.issn | 0012-1606 | - |
dc.identifier.issn | 1095-564X | - |
dc.identifier.uri | http://hdl.handle.net/2440/28134 | - |
dc.description.abstract | The Drosophilacyclin E (DmcycE) gene gives rise to two transcripts encoding proteins that differ at their N termini, DmcycEII and DmcycEI. This study presents the first in vivo dissection of Cyclin E function. Ectopic expression studies using N- and C-terminal deletions of DmcycEI revealed that a region of 322 residues surrounding the cyclin box is sufficient to induce entry of G₁-arrested larval eye imaginal disc cells into S phase. Ectopic expression of DmcycEI in the eye disc has been previously shown to drive anterior, but not posterior, G₁-phase cells within the morphogenetic furrow (MF) into S phase. Significantly, ectopic expression of DmcycEII and N-terminal deletions of DmcycEI were able to drive all G₁ cells within the morphogenetic furrow into S phase, while a C-terminal deletion of DmcycEI could not. The p21 homolog Dacapo was shown by yeast two-hybrid, coimmunolocalization, and in vivo functional studies not to be the mediator of the DmcycEI inhibition in posterior part of the MF. Taken together, these results reveal a novel zone within the posterior region of the MF where DmcycEI but not DmcycEII function is inhibited, and suggest that DmcycEII is a more potent inducer of S phase. | - |
dc.description.uri | http://www.sciencedirect.com/science/journal/00121606 | - |
dc.language.iso | en | - |
dc.publisher | Academic Press Inc | - |
dc.source.uri | http://dx.doi.org/10.1006/dbio.2001.0496 | - |
dc.subject | Cyclin E | - |
dc.subject | Dacapo | - |
dc.subject | G1-S phase | - |
dc.subject | cell cycle | - |
dc.subject | Drosophila | - |
dc.subject | development | - |
dc.title | Analysis of Drosophila Cyclin EI and II function during development: Identification of an inhibitory zone within the morphogenetic furrow of the eye imaginal disc that blocks the function of Cyclin EI but not Cyclin EII | - |
dc.type | Journal article | - |
dc.contributor.organisation | Centre for the Molecular Genetics of Development | - |
dc.identifier.doi | 10.1006/dbio.2001.0496 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 2 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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