Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/28151
Type: | Journal article |
Title: | Directed differentiation of pluripotent cells to neural lineages: homogeneous formation and differentiation of a neurectoderm population |
Author: | Rathjen, Joy R. Haines, Bryan Peter Hudson, Kathryn M. Nesci, Antonietta Dunn, Stephanie M. Rathjen, Peter David |
Citation: | Development, 2002; 129(11):2649-2661 |
Publisher: | Company of Biologists |
Issue Date: | 2002 |
ISSN: | 0950-1991 |
School/Discipline: | School of Molecular and Biomedical Science |
Organisation: | Centre for the Molecular Genetics of Development |
Abstract: | During embryogenesis the central and peripheral nervous systems arise from a neural precursor population, neurectoderm, formed during gastrulation. We demonstrate the differentiation of mouse embryonic stem cells to neurectoderm in culture, in a manner which recapitulates embryogenesis, with the sequential and homogeneous formation of primitive ectoderm, neural plate and neural tube. Formation of neurectoderm occurs in the absence of extraembryonic endoderm or mesoderm and results in a stratified epithelium of cells with morphology, gene expression and differentiation potential consistent with positionally unspecified neural tube. Differentiation of this population to homogeneous populations of neural crest or glia was also achieved. Neurectoderm formation in culture allows elucidation of signals involved in neural specification and generation of implantable cell populations for therapeutic use. |
Keywords: | Stem cells; Neurectoderm; Cell culture; Neural crest |
Published version: | http://dev.biologists.org/cgi/content/abstract/129/11/2649 |
Appears in Collections: | Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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