Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/28157
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dc.contributor.author | Ogunniyi, A. | - |
dc.contributor.author | Kotlarski, I. | - |
dc.contributor.author | Morona, R. | - |
dc.contributor.author | Manning, P. | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | FEMS Immunology and Medical Microbiology, 2002; 34(1):23-31 | - |
dc.identifier.issn | 0928-8244 | - |
dc.identifier.issn | 1574-695X | - |
dc.identifier.uri | http://hdl.handle.net/2440/28157 | - |
dc.description | The definitive version is available at www.blackwell-synergy.com | - |
dc.description.abstract | We have used a recombinant approach to characterise the B- and T-cell epitopes of FanC, the major subunit polypeptide of K99 (F5) fimbriae of enterotoxigenic Escherichia coli strains. This involved the fusion of FanC and its carboxy-terminal truncated derivatives to a reporter, the E. coli alkaline phosphatase (PhoA), generating stable, recombinant fusions. The B-cell epitopes of FanC were characterised by Western blotting of FanC::PhoA fusion proteins with a polyclonal mouse antiserum directed against K99 fimbrial antigen, and with a panel of monoclonal antibodies generated to the K99 antigen. An attempt to characterise the T-cell epitopes of the fimbrial subunit was made by standard in vitro T-cell proliferation assay. Our results suggest that the B-cell epitopes of FanC are likely to be continuous, with a potentially immunodominant epitope at the carboxy-terminus. However, T-cell proliferation assays with the FanC::PhoA fusion proteins did not indicate any immunodominant T-cell epitope(s). We hypothesise that fusion of FanC peptides to PhoA had resulted in altered folding of the peptides for antibody and T-cell recognition, highlighting the potential problems and drawbacks of the recombinant fusion technique in defining the epitopes of certain proteins. | - |
dc.description.statementofresponsibility | Abiodun D Ogunniyi, Ieva Kotlarski, Renato Morona, Paul A Manning | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Science BV | - |
dc.source.uri | http://www.blackwell-synergy.com/doi/abs/10.1111/j.1574-695X.2002.tb00599.x | - |
dc.subject | B-Lymphocytes | - |
dc.subject | T-Lymphocytes | - |
dc.subject | Fimbriae, Bacterial | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred BALB C | - |
dc.subject | Mice | - |
dc.subject | Escherichia coli | - |
dc.subject | Alkaline Phosphatase | - |
dc.subject | Cyclin-Dependent Kinases | - |
dc.subject | Escherichia coli Proteins | - |
dc.subject | Protein Subunits | - |
dc.subject | Recombinant Fusion Proteins | - |
dc.subject | Bacterial Toxins | - |
dc.subject | Antigens, Surface | - |
dc.subject | Epitopes | - |
dc.subject | Immunodominant Epitopes | - |
dc.subject | Epitope Mapping | - |
dc.subject | Protein Folding | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.title | Epitope analysis of the FanC subunit protein of the K99 (F5) fimbriae of enterotoxigenic Escherichia coli using a recombinant fusion technique | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1111/j.1574-695X.2002.tb00599.x | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Ogunniyi, A. [0000-0001-9308-5629] | - |
dc.identifier.orcid | Morona, R. [0000-0001-7009-7440] | - |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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