Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/28189
Type: | Journal article |
Title: | The Drosophila retained/dead ringer gene and ARID gene family function during development |
Author: | Shandala, T. Kortschak, R. Saint, R. |
Citation: | The International Journal of Developmental Biology, 2002; 46(4 Special Issue SI):423-430 |
Publisher: | Univ Basque Country Press |
Issue Date: | 2002 |
ISSN: | 0214-6282 1696-3547 |
Organisation: | Centre for the Molecular Genetics of Development |
Statement of Responsibility: | Tetyana Shandala, R. Daniel Kortschak and Robert Saint |
Abstract: | The recently discovered ARID family of proteins interact with DNA through a phylogenetically conserved sequence termed the A/T Interaction Domain (ARID). The retained/dead ringer (retn/dri) gene of Drosophila melanogaster is a founding member of the ARID gene family, and of the eARID subfamily. This subfamily exhibits an extended region of sequence similarity beyond the core ARID motif and a separate conserved domain termed the REKLES domain. retn/dri is involved in a range of developmental processes, including axis patterning and muscle development. The retn/dri ARID motif has been shown by in vitro studies to exhibit sequence-specific DNA binding activity. Here we demonstrate that the ARID domain is essential for the in vivo function of retn/dri during embryonic development by showing that a mutant form of RETN/DRI, deleted for part of the ARID domain and unable to bind DNA in vitro, cannot rescue the retn/dri mutant phenotype. In the presence of wild-type RETN/DRI this construct acts as a dominant negative, providing additional support for the proposal that RETN/DRI acts in a multiprotein complex. In contrast, we are yet to find an in vivo role for the REKLES domain, despite its clear evolutionary conservation. Finally, we have used germline clone analysis to reveal a requirement for retn/dri in the Drosophila preblastoderm syncytial mitoses. |
Keywords: | ARID motif gene regulation cell cycle control DNA binding proteins |
Description: | © UBC Press |
Published version: | http://www.ijdb.ehu.es/web/paper.php?doi=12141428 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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