Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/28221
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture |
Author: | Burrows, J. Nitsche, A. Bayly, B. Walker, E. Higgins, G. Kok, T. |
Citation: | BMC Microbiology, 2002; 2(12):www 1-www 7 |
Publisher: | BioMed Central Ltd. |
Issue Date: | 2002 |
ISSN: | 1471-2180 1471-2180 |
Statement of Responsibility: | Burrows Julie ; Nitsche Andreas ; Bayly Belinda ; Walker Elise ; Higgins Geoff ; Kok Tuckweng |
Abstract: | <h4>Background</h4>Prompt laboratory diagnosis of Herpes simplex virus (HSV) infection facilitates patient management and possible initiation of antiviral therapy. In our laboratory, which receives various specimen types for detection of HSV, we use enzyme immunoassay (EIA) for rapid detection and culture of this virus. The culture of HSV has traditionally been accepted as the diagnostic 'gold standard'. In this study, we compared the use of real time PCR (LightCycler) for amplification, detection and subtyping of specific DNA with our in-house developed rapid and culture tests for HSV.<h4>Results</h4>The LightCycler PCR (LC-PCR) detected and subtyped HSV in 99% (66/67) of HSV positive specimens, compared to 81% (54/67) by rapid antigen EIA or 57% (36/63) by culture. A specimen was considered positive when two or more tests yielded HSV identifications or was culture positive. Discordant results were confirmed with an in-house developed PCR-ELISA or DNA sequence analysis. The typing results obtained with the LC-PCR and by culture amplified test were completely concordant.<h4>Conclusions</h4>This study showed that the LC-PCR provided a highly sensitive test for simultaneous detection and subtyping of HSV in a single reaction tube. In addition to increased sensitivity, the LightCycler PCR provided reduced turn-around-times (2 hours) when compared to enzyme immunoassay (4 hours) or culture (4 days). |
Keywords: | Tumor Cells, Cultured Humans Simplexvirus Herpesvirus 1, Human Herpesvirus 2, Human DNA, Viral Immunoenzyme Techniques Sensitivity and Specificity Polymerase Chain Reaction Sequence Analysis, DNA |
DOI: | 10.1186/1471-2180-2-12 |
Published version: | http://dx.doi.org/10.1186/1471-2180-2-12 |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.