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https://hdl.handle.net/2440/3100
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Type: | Journal article |
Title: | Inhibition of cytokine-induced fractalkine production by bacterial invasion of human-dermal fibroblasts |
Author: | Fahy, O. Coates, N. McColl, S. |
Citation: | Laboratory Investigation, 2003; 83(5):721-730 |
Publisher: | Lippincott Williams & Wilkins |
Issue Date: | 2003 |
ISSN: | 0023-6837 1530-0307 |
Statement of Responsibility: | Olivier Luc Fahy, Nicholas John Coates and Shaun Reuss McColl |
Abstract: | Fractalkine (FKN/CX3CL1) is an atypical chemokine, for which a major biological function has not yet emerged. However, recent data suggest a role in immune responses in the skin. In this study, we analyzed fractalkine (FKN) secretion by human-dermal fibroblasts after exposure to pro-inflammatory cytokines or to invasive and noninvasive strains of Escherichia coli. Incubation of fibroblasts with TNF- and IL-1 induced a delayed expression of soluble FKN, compared with the rapid secretion of other chemokines including IL-8 (CXCL8), monocyte chemotactic protein-1 (CCL2), and RANTES (regulated upon activation, normal T cell expressed and secreted; CCL5). TNF- and IFN- gamma were more potent at inducing FKN secretion than was IL-1. Very little FKN was detected on the cell surface. FKN was not detected after incubation with the bacteria, regardless of the strain used. In contrast, both invasive and noninvasive E. coli triggered the release of IL-8 and monocyte chemotactic protein-1 in a dose-response manner, whereas RANTES was produced only in response to the invasive strain. Finally, incubation of fibroblasts with the invasive strain of E. coli inhibited TNF-– and IFN-–induced production of FKN. These results demonstrate for the first time that human-dermal fibroblasts express FKN, and that the characteristics of FKN secretion are distinct from those of other chemokines produced by these cells during immune responses in the dermis. In addition, our data indicate that bacterial invasion of dermal fibroblasts actively modulates FKN expression. |
Keywords: | Cells, Cultured Fibroblasts Skin Humans Escherichia coli Tumor Necrosis Factor-alpha Membrane Proteins Chemokines, CX3C Interleukin-1 Reverse Transcriptase Polymerase Chain Reaction Chemokine CX3CL1 |
Description: | © 2007 United States and Canadian Academy of Pathology |
DOI: | 10.1097/01.LAB.0000069518.49544.B8 |
Published version: | http://www.nature.com/labinvest/journal/v83/n5/abs/3780659a.html |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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