Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/3166
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Molecular characterization of duck hepatitis B virus isolates from South African ducks |
Author: | Mangisa, N. Smuts, H. Kramvis, A. Linley, C. Skelton, M. Tucker, T. de la M Hall, P. Kahn, D. Jilbert, A. Kew, M. |
Citation: | Virus Genes, 2004; 28(2):179-186 |
Publisher: | Kluwer Academic Publ |
Issue Date: | 2004 |
ISSN: | 0920-8569 1572-994X |
Statement of Responsibility: | Nomathibane P. Mangisa, Heidi E. Smuts, Anna Kramvis, C. Wendy Linley, Michelle Skelton, Timothy J. Tucker, Pauline De La M. Hall, Del Kahn, Allison R. Jilbert and Michael C. Kew |
Abstract: | The objective of the study was to characterize the genome of duck hepatitis B virus (DHBV) isolates from South African Pekin ducks. Duck serum and liver samples were collected from two commercial duck farms from geographically distinct regions of South Africa. In total, 498 duck serum samples were tested for the presence of DHBV DNA using either sub-genomic or full-length polymerase chain reaction (PCR) assays. The overall prevalence of DHBV infection in South African ducks was 47%. In addition, 30% of 59 liver tissues tested were DHBV DNA-positive. Six randomly selected serum or liver samples were used to clone and sequence the genomes of the South African DHBV strains. All six isolates had DHBV genomes of 3,021 nucleotides with three characteristic overlapping reading frames encoding the polymerase, surface and core gene products. No X-like gene with a traditional start codon was found. Following phylogenetic analysis, the South African DHBV isolates clustered with DHBV isolates from other ldquoWesternrdquo countries, including United States of America, Canada, Germany and India. On translation of the open reading frames, the South African isolates were found to share signature amino acids in the polymerase and surface genes with the ldquoWesternrdquo country isolates as opposed to those of Chinese DHBV isolates. |
Keywords: | Chinese and Western isolates cloning phylogenetic analysis sequencing |
Rights: | © 2004 Kluwer Academic Publishers |
DOI: | 10.1023/B:VIRU.0000016856.56499.32 |
Published version: | http://dx.doi.org/10.1023/b:viru.0000016856.56499.32 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.