Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/34043
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Vitrification of mouse oocytes using a nylon loop. |
Author: | Lane, M. Gardner, D. |
Citation: | Molecular Reproduction and Development, 2001; 58(3):342-347 |
Publisher: | Wiley-Liss |
Issue Date: | 2001 |
ISSN: | 1040-452X 1098-2795 |
Statement of Responsibility: | Michelle Lane, David K. Gardner |
Abstract: | Cryopreservation of mouse oocytes was improved by the use of ultra-rapid vitrification using a nylon loop of 0.5 mm diameter. Oocytes that were vitrified using the loop survived at high rates and were fertilized following a small hole being made in the zona pellucida (69.8%) and developed to the blastocyst stage in culture (67.4%) at similar rates to that of oocytes that were not cryopreserved. Blastocysts resulting from oocytes vitrified using the nylon loop had similar development of the inner cell mass and trophectoderm as blastocysts from non-cryopreserved oocytes. In contrast, oocytes that were cryopreserved using a slow-freezing protocol where most of the Na+ is replaced with choline had lower rates of fertilization (39.5%), reduced development to the blastocyst stage (25.7%), and blastocysts had reduced development of the inner cell mass. Blastocysts derived from oocytes that were vitrified with the nylon loop were able to implant (88.0%) and develop into fetuses (56.5%) at significantly higher rates compared to blastocysts derived from oocytes that were slow-frozen (52.4 and 26.2%, respectively). Vitrification of mouse oocytes using the nylon loop results in the retention of viability of the oocytes and subsequent embryos. |
Keywords: | cryopreservation development embryo IVF viability |
Description: | The definitive version may be found at www.wiley.com |
DOI: | 10.1002/1098-2795(200103)58:3<342::AID-MRD13>3.0.CO;2-X |
Published version: | http://www3.interscience.wiley.com/cgi-bin/abstract/76510540/ABSTRACT |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.