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https://hdl.handle.net/2440/39289
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Type: | Journal article |
Title: | Expression of bone matrix protein mRNAs by primary and cloned cultures of the regenerative phenotype of human periodontal fibroblasts |
Author: | Ivanovski, S. Haase, H. Bartold, P. |
Citation: | Journal of Dental Research, 2001; 80(7):1665-1671 |
Publisher: | Inter Amer Assoc Dental Research |
Issue Date: | 2001 |
ISSN: | 0022-0345 1544-0591 |
Statement of Responsibility: | S. Ivanovski, H. R. Haase and P. M. Bartold |
Abstract: | The successful regeneration of periodontal tissues is dependent, in part, on the ability of cells to reconstitute the mineralized tissues of cementum and bone. The aim of the present study was to characterize regeneration-associated cells in terms of their ability to express mineralized tissue macromolecules. Following guided tissue regeneration, cell cultures were established from regenerating tissue, periodontal ligament, and gingiva. Additionally, these cells were transfected, and single-cell-derived clones were established. Following treatment with platelet-derived growth factor-BB and insulin-derived growth factor-1, the presence of mRNA for alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin, and bone morphogenetic proteins-2 and -4 was assessed. The three cell types expressed similar mRNA levels for alkaline phosphatase, bone morphogenetic protein-2, and bone morphogenetic protein-4, whereas the expression of osteopontin, osteocalcin, and bone sialoprotein was greater in the periodontal ligament and regenerating tissue fibroblasts compared with the gingival fibroblasts. The two growth factors did not affect the expression of any of the genes. This study has identified markers that correlate with the known ability of periodontal ligament and regenerating tissue-derived fibroblasts to facilitate regeneration of the mineralized tissues of the periodontium. |
Keywords: | Bone Matrix Cells, Cultured Clone Cells Fibroblasts Stem Cells Periodontal Ligament Humans Alkaline Phosphatase Sialoglycoproteins Osteocalcin Bone Morphogenetic Proteins Extracellular Matrix Proteins RNA, Messenger Histocytochemistry Analysis of Variance Reverse Transcriptase Polymerase Chain Reaction Regeneration Bone Regeneration Phenotype Osteopontin Integrin-Binding Sialoprotein |
Description: | Copyright © 2001 by International & American Associations for Dental Research Online Journals |
DOI: | 10.1177/00220345010800071301 |
Published version: | http://jdr.iadrjournals.org/cgi/content/abstract/80/7/1665 |
Appears in Collections: | Aurora harvest 6 Dentistry publications |
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