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https://hdl.handle.net/2440/3974
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Type: | Journal article |
Title: | Receptor activator NF kB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis |
Author: | Crotti, T. Smith, M. Hirsch, R. Soukoulis, S. Weedon, H. Capone, M. Ahern, M. Haynes, D. |
Citation: | Journal of Periodontal Research, 2003; 38(4):380-387 |
Publisher: | Munksgaard Int Publ Ltd |
Issue Date: | 2003 |
ISSN: | 0022-3484 1600-0765 |
Statement of Responsibility: | Tania Crotti, Malcolm D. Smith, Robert Hirsch, Steven Soukoulis, Helen Weedon, Maria Capone, Michael J. Ahern, David Haynes |
Abstract: | Objectives and background: This study investigated the expression of key mediators that regulate differentiation of osteoclasts, receptor activator of nuclear factor κB ligand (RANKL), and its natural inhibitor, osteoprotegerin (OPG), in periodontitis. We aimed to compare the levels of the RANKL and OPG in the granulomatous tissue adjacent to areas of alveolar bone loss from patients with periodontitis to that present in tissue from patients without periodontitis. In addition, we aimed to determine the types of cells expressing these factors in these tissues and to demonstrate the expression of the osteoclastic markers, RANK and tartrate-resistant acid phosphatase (TRAP), in periodontitis. Materials and methods: Frozen biopsy specimens were analysed using specific monoclonal antibodies and were evaluated by semiquantitative analysis and digital image analysis to compare levels of RANKL and OPG protein expression. Double labelling of frozen sections with antibodies to different cell lineage specific markers was used to determine the types of cells expressing these proteins. In situ hybridization was used to detect cells expressing RANK mRNA. Results: Semiquantitative image analysis demonstrated that significantly higher levels of RANKL protein (P < 0.05) were expressed in the periodontitis tissue. Conversely, OPG protein was significantly lower (P < 0.05) in the periodontitis tissues. RANKL protein was associated with lymphocytes and macrophages. OPG protein was associated with endothelial cells in both tissues. Many leukocytes expressing RANK mRNA and TRAP were observed in periodontitis tissues. Conclusion: The change in the levels of these key regulators of osteoclast differentiation may play a major role in the bone loss seen in periodontitis. |
Keywords: | Endothelium, Vascular Leukocytes, Mononuclear Osteoclasts Humans Alveolar Bone Loss Periodontitis Acid Phosphatase Isoenzymes Glycoproteins Carrier Proteins NF-kappa B Membrane Glycoproteins Receptors, Tumor Necrosis Factor Receptors, Cytoplasmic and Nuclear Ligands Cell Differentiation Gene Expression Regulation Adolescent Adult Aged Middle Aged Female Male RANK Ligand Receptor Activator of Nuclear Factor-kappa B Osteoprotegerin Biomarkers Tartrate-Resistant Acid Phosphatase |
Description: | The definitive version is available at www.blackwell-synergy.com Article first published online: 26 JUN 2003 |
Rights: | Copyright © Blackwell Munksgaard Ltd |
DOI: | 10.1034/j.1600-0765.2003.00615 |
Published version: | http://www.blackwell-synergy.com/doi/abs/10.1034/j.1600-0765.2003.00615 |
Appears in Collections: | Aurora harvest 6 Dentistry publications Pathology publications |
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