The construction of an almond linkage map using morphological and microsatellite markers on 'Nonpariel' x 'Lauranne' population.

Abstract

A set of 110 microsatellite primers has recently developed from almond, peach and apricot enriched genomic libraries was used to saturate a previously developed linkage map derived from “Lauranne X Nonpareil “ F1 progeny. Forty-six microsatellites showed polymorphic patterns, seven have been mapped and detected nine, ten and eight linkage groups for Nonpareil, Lauranne and the integrated map, respectively. Other polymorphic markers will be screened on entire population to achieve eight linkage groups. A number of morphological traits such as shell, testa and kernel characteristics and percentage of doubles were measured and analyzed. Most of these traits will be mapped as quantitative trait loci (QTL). Bacterial spot assay have been carried out and preliminary results show remarkable differences within the segregating population.