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https://hdl.handle.net/2440/42895
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Type: | Journal article |
Title: | Fungal allergens induce cathelicidin LL-37 expression in chronic rhinosinusitis patients in a nasal explant model |
Author: | Ooi, E. Wormald, P. Carney, S. James, C. Tan, L. |
Citation: | American Journal of Rhinology and Allergy, 2007; 21(3):367-372 |
Publisher: | Ocean Side Publications Inc |
Issue Date: | 2007 |
ISSN: | 1945-8924 1050-6586 |
Statement of Responsibility: | Ooi, Eng Hooi; Wormald, Peter-John; Carney, A. Simon; James, Craig Lloyd; Tan, Lor Wai |
Abstract: | <h4>Background</h4>Fungus is thought to play an important role in some subgroups of chronic rhinosinusitis (CRS) patients with eosinophilic mucus (EMCRS). The cathelicidin LL-37 is an important innate defense peptide with antimicrobial activity but its responses in CRS and EMCRS patients have not been established. We investigated the innate immune responses of LL-37 in nasal tissue from CRS and EMCRS patients to fungal allergen challenge.<h4>Methods</h4>The levels of LL-37 produced by nasal tissue and secreted in response to fungal allergen challenge were determined by a nasal tissue explant in vitro model. LL-37 mRNA and protein levels were quantified by real-time reverse-transcriptase-polymerase chain reaction and immunoassay methods.<h4>Results</h4>LL-37 mRNA expression in CRS, but not EMCRS patients, is significantly upregulated by Aspergillus (mean fourfold increase) and Alternaria (mean sixfold increase) extracts in a dose-response manner (p < 0.001). LL-37 peptide levels in the nasal tissue from CRS patients are increased in response to Alternaria (p < 0.05). In contrast, with EMCRS patients, the expression of LL-37 peptide in nasal tissue is increased with Aspergillus (p < 0.001) but is reduced with Alternaria. We also observed a trend where levels of secreted LL-37 were decreased with higher doses of Alternaria and Aspergillus extracts.<h4>Conclusion</h4>LL-37 is significantly up-regulated at the mRNA and protein level in CRS patients in response to fungal allergens. However, EMCRS patients do not show increased LL-37 at either the mRNA or the protein level in response to Alternaria. |
Keywords: | Paranasal Sinuses Cells, Cultured Humans Fungi Alternaria Aspergillus Sinusitis Rhinitis Lipopolysaccharides Antimicrobial Cationic Peptides RNA, Messenger Allergens Biopsy Up-Regulation Multigene Family Cathelicidins |
DOI: | 10.2500/ajr.2007.21.3025 |
Description (link): | http://www.ncbi.nlm.nih.gov/pubmed/17621825 |
Published version: | http://dx.doi.org/10.2500/ajr.2007.21.3025 |
Appears in Collections: | Aurora harvest Surgery publications |
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