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https://hdl.handle.net/2440/43298
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Type: | Journal article |
Title: | A Drosophila overexpression screen for modifiers of Rho signalling in cytokinesis |
Author: | Gregory, S. Shandala, T. O'Keefe, L. Jones, L. Murray, M. Saint, R. |
Citation: | Fly, 2007; 1(1):13-23 |
Publisher: | Landes Bioscience |
Issue Date: | 2007 |
ISSN: | 1933-6934 1933-6942 |
Organisation: | Centre for the Molecular Genetics of Development |
Statement of Responsibility: | Stephen L. Gregory, Tetyana Shandala, Louise O'Keefe, Lynn Jones, Michael J. Murray and Robert Saint |
Abstract: | To identify genes that modulate Rho signalling during cytokinesis we tested the effect of overexpressing a set of 2190 genes on an eye phenotype caused by defective Rho activation. The resulting 112 modifier loci fell into three main classes: cell cycle genes, signalling effectors and metabolic enzymes. We developed a further series of genetic tests to refine the interactors into those most likely to modify Rho signalling during cytokinesis. In addition to a number of genes previously implicated in the Rho pathway during cytokinesis, we identified four novel primary candidates: cdc14, Pitslre, PDK1 and thread/diap1. cdc14 orthologs have, however, been implicated in cytokinesis in other organisms, as have molecules related to Thread/Diap1. The identification of several modifiers that are genetically redundant paralogs highlights the ability of overexpression screens to identify genes that are refractory to traditional loss-of-function approaches. Overexpression screens and sensitized phenotypes, therefore, may help identify the many factors that are expected to be involved in cytokinesis but have not been discovered by previous genetic screens. |
Keywords: | Eye Animals Drosophila rhoA GTP-Binding Protein Guanine Nucleotide Exchange Factors Drosophila Proteins Genetic Markers Signal Transduction Cytokinesis RNA Interference Wings, Animal |
Description: | © 2000-2008 Landes Bioscience |
DOI: | 10.4161/fly.3806 |
Published version: | http://www.landesbioscience.com/journals/fly/article/3806 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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